Detailed information of TA system TA206539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2257079..2257608
• Replicon: chromosome
• Accession: NZ_CP076839
• Organism: Staphylococcus aureus strain 55-100-016

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KRH48_RS10745
• Protein ID: WP_000621175.1
• Coordinates: 2257246..2257608 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KRH48_RS10740
• Protein ID: WP_000948331.1
• Coordinates: 2257079..2257249 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRH48_RS10710 (KRH48_10710)	2252115..2252675	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
KRH48_RS10715 (KRH48_10715)	2252884..2253363	+	480	WP_001287081.1	hypothetical protein	-
KRH48_RS10720 (KRH48_10720)	2253356..2254933	+	1578	WP_001294650.1	PH domain-containing protein	-
KRH48_RS10725 (KRH48_10725)	2254926..2255417	+	492	WP_001286980.1	PH domain-containing protein	-
KRH48_RS10730 (KRH48_10730)	2255421..2255780	+	360	WP_000581197.1	holo-ACP synthase	-
KRH48_RS10735 (KRH48_10735)	2255846..2256994	+	1149	WP_001281139.1	alanine racemase	-
KRH48_RS10740 (KRH48_10740)	2257079..2257249	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KRH48_RS10745 (KRH48_10745)	2257246..2257608	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KRH48_RS10750 (KRH48_10750)	2257958..2258959	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KRH48_RS10755 (KRH48_10755)	2259078..2259404	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KRH48_RS10760 (KRH48_10760)	2259406..2259885	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
KRH48_RS10765 (KRH48_10765)	2259860..2260630	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T206539 WP_000621175.1 NZ_CP076839:2257246-2257608 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T206539 NZ_CP102480:3679532-3679786 [Nisaea acidiphila]
ATGAAGCTCGTCTTCCACGAGCGGGCCTGGGAGGACTACCTCCATTGGCAGAAGACGGACCGGAAGCTGCATCAGCGTCT
GAATGCCCTGATCCGCGAATGCACGCGGACGCCCTTCGAGGGGACCGGCAAGCCGGAACCTTTGCGGGGGCCGCTCTCCG
GCTGGTGGTCGCGGCGGCTGACGCTGGAGCACCGGCTGGTCTACCGCGTCCACGAGGACCAGCTGCAGATCGCGCAGTGC
CGGTATCATTACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT206539 WP_000948331.1 NZ_CP076839:2257079-2257249 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT206539 NZ_CP102480:3679281-3679535 [Nisaea acidiphila]
ATGCGCACCAAATCCTACAGCGACCTCCGGCGAAACCTCGCGGCCACGCTCGACAGCGTGACGGCGGATCATGAGCCGGT
GATCATCACGCGGGACCGGGGCAAGCCGGCGGCCGTGCTGATGTCGCTTGAGGATTTCGCCTCCTACGAGGAAACCCGCT
ACCTGCTGCGCAGCCCGGAGAATGCGGACCGGCTGATCGAGGCGGTGAGCGCGCTCGAAGCCGGGGCCGGACGGGAAGAA
CAGCTCGAGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1