Detailed information of TA system TA020653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 924141..924748
• Replicon: chromosome
• Accession: NC_003062
• Organism: Agrobacterium tumefaciens str. C58

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q7D0B7
• Locus tag: ATU_RS04610
• Protein ID: WP_010971265.1
• Coordinates: 924141..924476 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A9CJP8
• Locus tag: ATU_RS04615
• Protein ID: WP_010971266.1
• Coordinates: 924473..924748 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATU_RS04595 (AGR_C_1696)	920246..922441	-	2196	WP_035256415.1	penicillin-binding protein	-
ATU_RS04600 (AGR_C_1698)	922698..923177	+	480	WP_010971263.1	YcgN family cysteine cluster protein	-
ATU_RS04605 (AGR_C_1700)	923289..924113	+	825	WP_010971264.1	class D beta-lactamase	-
ATU_RS04610 (AGR_C_1701)	924141..924476	-	336	WP_010971265.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ATU_RS04615 (AGR_C_1704)	924473..924748	-	276	WP_010971266.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ATU_RS04620 (AGR_C_1706)	924856..925464	+	609	WP_010971267.1	class I SAM-dependent methyltransferase	-
ATU_RS04625 (AGR_C_1708)	925640..928225	+	2586	WP_035256421.1	heavy metal translocating P-type ATPase	-
ATU_RS04630 (AGR_C_1709)	928222..928644	+	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
ATU_RS04635	928697..928897	+	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
ATU_RS04640 (AGR_C_1711)	928967..929233	+	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ATU_RS04645 (AGR_C_1712)	929233..929592	+	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (23-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-67)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12991.86 Da
• Isoelectric Point: 10.0059

>T20653 WP_010971265.1 NC_003062:c924476-924141 [Agrobacterium fabrum str. C58]
VTNKKDHGKDAALKRATLPRRSDFTKQFIKDWQRLNNSGRYDMVRLKEIMLLLIANGAPLPTQFRDHELTGDWRDHRECH
VGGDFLLIYTVDEKQNLLIFTRAGTHAELFR

Nucleotide

• Length: 336 bp

>T20653 NC_003062:c924476-924141 [Agrobacterium fabrum str. C58]
GTGACGAATAAGAAAGATCACGGAAAAGACGCGGCATTGAAGCGAGCCACCCTGCCGCGCAGGTCGGATTTCACCAAGCA
ATTTATCAAAGACTGGCAACGGCTGAACAATTCCGGGCGTTACGACATGGTGAGACTGAAGGAGATCATGCTCCTTCTTA
TCGCCAACGGCGCGCCGTTACCGACGCAGTTTCGTGACCATGAACTGACCGGAGACTGGCGCGATCACCGGGAATGCCAT
GTCGGCGGGGATTTTCTGTTGATCTACACAGTCGATGAGAAGCAGAACCTTCTCATCTTCACCCGCGCGGGAACGCACGC
CGAGCTTTTCAGGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9918.40 Da
• Isoelectric Point: 6.3350

>AT20653 WP_010971266.1 NC_003062:c924748-924473 [Agrobacterium fabrum str. C58]
MTANAYVRARIDQTLKDDATAVLDRLGLTVSDVMRMMLTRIAREKALPIELTQPNAETLAAIEEARAIAAAGRNRFGTSE
ALFEALDAGKR

Nucleotide

• Length: 276 bp

>AT20653 NC_003062:c924748-924473 [Agrobacterium fabrum str. C58]
ATGACCGCAAATGCTTATGTAAGAGCGCGCATCGATCAGACACTCAAGGACGACGCGACGGCCGTGCTGGACCGCCTGGG
ACTGACTGTTTCAGATGTCATGCGAATGATGCTGACGCGCATTGCCCGAGAAAAAGCTCTGCCGATAGAACTGACACAAC
CAAACGCTGAAACCCTTGCCGCGATTGAAGAAGCGCGCGCCATCGCGGCGGCCGGGCGCAATCGTTTCGGCACGTCTGAA
GCCTTGTTTGAGGCGCTGGACGCTGGCAAACGGTGA

Structures

Toxin

• AlphaFold DB: Q7D0B7

Antitoxin

• AlphaFold DB: A9CJP8