Detailed information of TA system TA020652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 670257..670737
• Replicon: chromosome
• Accession: NC_003062
• Organism: Agrobacterium tumefaciens str. C58

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A9CK22
• Locus tag: ATU_RS03315
• Protein ID: WP_010971059.1
• Coordinates: 670257..670526 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A9CK21
• Locus tag: ATU_RS03320
• Protein ID: WP_010971060.1
• Coordinates: 670510..670737 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATU_RS03285 (AGR_C_1197)	665308..666084	-	777	WP_010971054.1	L,D-transpeptidase	-
ATU_RS03290 (AGR_C_1200)	666323..666952	+	630	WP_010971055.1	DNA-3-methyladenine glycosylase I	-
ATU_RS03295 (AGR_C_1201)	666949..667392	+	444	WP_010972617.1	hypothetical protein	-
ATU_RS03300 (AGR_C_1202)	667418..668461	-	1044	WP_035256280.1	YeiH family protein	-
ATU_RS03305 (AGR_C_1204)	668543..669460	+	918	WP_010971057.1	LysR family transcriptional regulator	-
ATU_RS03310 (AGR_C_1205)	669486..670250	-	765	WP_010971058.1	hypothetical protein	-
ATU_RS03315 (AGR_C_1206)	670257..670526	-	270	WP_010971059.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ATU_RS03320 (AGR_C_1208)	670510..670737	-	228	WP_010971060.1	ribbon-helix-helix protein, CopG family	Antitoxin
ATU_RS03325 (AGR_C_1210)	670894..672417	+	1524	WP_010971061.1	histidine--tRNA ligase	-
ATU_RS03330 (AGR_C_1212)	672422..672790	+	369	WP_010971062.1	VOC family protein	-
ATU_RS03335 (AGR_C_1214)	672807..673934	+	1128	WP_010971063.1	ATP phosphoribosyltransferase regulatory subunit	-
ATU_RS03340 (AGR_C_1215)	673931..674623	+	693	WP_010971064.1	ATP phosphoribosyltransferase	-
ATU_RS03345 (AGR_C_1217)	674688..675134	-	447	WP_006313161.1	DoxX family protein	-
ATU_RS03350 (AGR_C_1218)	675290..675724	-	435	WP_010971066.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10746.46 Da
• Isoelectric Point: 9.9414

>T20652 WP_010971059.1 NC_003062:c670526-670257 [Agrobacterium fabrum str. C58]
VIWTIEYHTLVQKEMRKINPEVRRRIRSFLHERLAALDDPRQIGATLQGSELGNFWRYRVGDYRIICDIQDQKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T20652 NC_003062:c670526-670257 [Agrobacterium fabrum str. C58]
GTGATCTGGACAATTGAATATCACACGCTTGTGCAGAAAGAGATGCGCAAAATAAATCCGGAAGTGCGCCGGCGCATCCG
CAGTTTTCTCCATGAAAGGCTGGCCGCACTGGACGACCCGCGCCAGATCGGCGCTACACTGCAAGGTTCCGAACTCGGAA
ATTTCTGGCGTTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCAAAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8937.97 Da
• Isoelectric Point: 4.7254

>AT20652 WP_010971060.1 NC_003062:c670737-670510 [Agrobacterium fabrum str. C58]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKIISAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT20652 NC_003062:c670737-670510 [Agrobacterium fabrum str. C58]
ATGAGCAAACAGACGGCCATTCGCCTGCCGGACGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGGACCGGCCGCAC
ATCCGCCTATTATATCCGCGAGGCGATCGAGAAGCATATCGAGGATATGGAAGACCTCTATCTTGCCGAAGAGGCGACGC
GGCGCATTCAGCGCGGAGAATCGAAGATTATAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Structures

Toxin

• AlphaFold DB: A9CK22

Antitoxin

• AlphaFold DB: A9CK21