Detailed information of TA system TA206516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 197025..197611
• Replicon: chromosome
• Accession: NZ_CP076838
• Organism: Leclercia pneumoniae strain 49125

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0M3F019
• Locus tag: KQ929_RS00935
• Protein ID: WP_040078242.1
• Coordinates: 197243..197611 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: KQ929_RS00930
• Protein ID: WP_207292542.1
• Coordinates: 197025..197246 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ929_RS00905 (KQ929_00905)	192103..193212	+	1110	WP_040078235.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
KQ929_RS00910 (KQ929_00910)	193259..194185	+	927	WP_040078237.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KQ929_RS00915 (KQ929_00915)	194182..195459	+	1278	WP_207292543.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
KQ929_RS00920 (KQ929_00920)	195456..196223	+	768	WP_046886787.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KQ929_RS00925 (KQ929_00925)	196225..196938	+	714	WP_046886786.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KQ929_RS00930 (KQ929_00930)	197025..197246	+	222	WP_207292542.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KQ929_RS00935 (KQ929_00935)	197243..197611	+	369	WP_040078242.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KQ929_RS00940 (KQ929_00940)	198083..199399	+	1317	WP_040078243.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KQ929_RS00945 (KQ929_00945)	199516..200403	+	888	WP_040078244.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KQ929_RS00950 (KQ929_00950)	200400..201245	+	846	WP_207292541.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KQ929_RS00955 (KQ929_00955)	201247..202317	+	1071	WP_040078246.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	195456..203057	7601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-112)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13650.95 Da
• Isoelectric Point: 6.7177

>T206516 WP_040078242.1 NZ_CP076838:197243-197611 [Leclercia pneumoniae]
MTLQFISAEEIVLFHDKLLSVTPGVAGMPDPGRAEALLYRVLNKYEYEGVTDVWMLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLKANPDYVDMTVEAAAGRLTLDEVVQRLRC

Nucleotide

• Length: 369 bp

>T206516 NZ_CP102473:774100-774417 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTATTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8041.04 Da
• Isoelectric Point: 5.6663

>AT206516 WP_207292542.1 NZ_CP076838:197025-197246 [Leclercia pneumoniae]
MRTMNYSEARQNLASALESAAAGTPVTISRRGHKSAVIISVEEFERYQTAKLDAEFDAIMGIHGSEIRELADK

Nucleotide

• Length: 222 bp

>AT206516 NZ_CP102473:774421-774720 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
TTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AAGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTCAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0M3F019