20649

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacT-ataR/DUF1778(antitoxin)
Location 2842713..2843479 Replicon chromosome
Accession NC_003047
Organism Sinorhizobium meliloti 1021

Toxin (Protein)


Gene name tacT Uniprot ID H0G6D3
Locus tag SM_RS13490 Protein ID WP_003533488.1
Coordinates 2842997..2843479 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID Q92MI4
Locus tag SM_RS13485 Protein ID WP_010970089.1
Coordinates 2842713..2842997 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM_RS13470 (SMc00743) 2838183..2839571 + 1389 WP_003533497.1 lipopolysaccharide biosynthesis protein -
SM_RS13475 (SMc00742) 2839575..2840846 + 1272 WP_010970087.1 GNAT family N-acetyltransferase -
SM_RS13480 (SMc00741) 2840862..2842508 - 1647 WP_010970088.1 AMP-binding protein -
SM_RS13485 (SMc00740) 2842713..2842997 + 285 WP_010970089.1 DUF1778 domain-containing protein Antitoxin
SM_RS13490 (SMc00739) 2842997..2843479 + 483 WP_003533488.1 GNAT family N-acetyltransferase Toxin
SM_RS13500 (SMc00738) 2843944..2844453 + 510 WP_010970090.1 disulfide bond formation protein B -
SM_RS13505 (SMc00737) 2844485..2845042 - 558 WP_003533486.1 HNH endonuclease -
SM_RS13510 (SMc00736) 2845148..2845795 - 648 WP_010970091.1 DNA-3-methyladenine glycosylase -
SM_RS13515 (SMc00735) 2845948..2846844 + 897 WP_010970092.1 tRNA glutamyl-Q(34) synthetase GluQRS -
SM_RS13520 (SMc00734) 2846863..2847738 - 876 WP_010970093.1 YihY/virulence factor BrkB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-142)

Antitoxin

Vibrio phage ICP1, Orf50

(15-79)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17463.88 Da        Isoelectric Point: 7.3827

>T20649 WP_003533488.1 NC_003047:2842997-2843479 [Sinorhizobium meliloti 1021]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Download         Length: 483 bp

>T20649 NC_003047:2842997-2843479 [Sinorhizobium meliloti 1021]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10741.10 Da        Isoelectric Point: 7.3175

>AT20649 WP_010970089.1 NC_003047:2842713-2842997 [Sinorhizobium meliloti 1021]
MATKSSADTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Download         Length: 285 bp

>AT20649 NC_003047:2842713-2842997 [Sinorhizobium meliloti 1021]
ATGGCCACGAAATCGTCGGCTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H0G6D3

Antitoxin

Source ID Structure
AlphaFold DB Q92MI4

References