206487

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 4626..5214 Replicon plasmid p1UC23022
Accession NZ_CP076813
Organism Acinetobacter baumannii strain UC23022

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag KQ253_RS20630 Protein ID WP_000438826.1
Coordinates 4927..5214 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag KQ253_RS20625 Protein ID WP_001983304.1
Coordinates 4626..4940 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ253_RS20600 (KQ253_20505) 1..924 + 924 WP_033107831.1 replication initiation protein RepM -
KQ253_RS20605 (KQ253_20510) 1009..1542 + 534 WP_033107830.1 plasmid replication DNA-binding protein -
KQ253_RS20610 (KQ253_20515) 1816..2292 - 477 WP_033107829.1 hypothetical protein -
KQ253_RS20615 (KQ253_20520) 2462..3166 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KQ253_RS20620 (KQ253_20525) 3218..4498 - 1281 Protein_4 TonB-dependent receptor ZnuD2 -
KQ253_RS20625 (KQ253_20530) 4626..4940 - 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
KQ253_RS20630 (KQ253_20535) 4927..5214 - 288 WP_000438826.1 BrnT family toxin Toxin
KQ253_RS20635 (KQ253_20540) 5555..5716 + 162 WP_153603871.1 hypothetical protein -
KQ253_RS20640 (KQ253_20545) 5750..6454 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KQ253_RS20645 (KQ253_20550) 6501..6692 - 192 WP_253526537.1 hypothetical protein -
KQ253_RS20650 (KQ253_20555) 6710..6976 - 267 WP_006581723.1 hypothetical protein -
KQ253_RS20655 (KQ253_20560) 7186..8622 + 1437 WP_033107832.1 MobQ family relaxase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..9197 9197
- inside IScluster/Tn - - 2462..6454 3992


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T206487 WP_000438826.1 NZ_CP076813:c5214-4927 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T206487 NZ_CP102441:1017693-1017983 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT206487 WP_001983304.1 NZ_CP076813:c4940-4626 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT206487 NZ_CP102441:1017397-1017696 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References