Detailed information of TA system TA020647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2199475..2200129
• Replicon: chromosome
• Accession: NC_003047
• Organism: Sinorhizobium meliloti 1021

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: SM_RS10460
• Protein ID: WP_003530010.1
• Coordinates: 2199475..2199663 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: SM_RS10465
• Protein ID: WP_010969659.1
• Coordinates: 2199668..2200129 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM_RS10445 (SMc04208)	2196041..2197351	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
SM_RS10450 (SMc04209)	2197376..2198347	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
SM_RS10455 (SMc04267)	2198528..2199298	-	771	WP_010969658.1	hypothetical protein	-
SM_RS10460 (SMc04441)	2199475..2199663	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
SM_RS10465 (SMc04269)	2199668..2200129	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SM_RS10475 (SMc04271)	2200448..2201365	-	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
SM_RS10480 (SMc04272)	2201542..2202291	-	750	WP_010969660.1	NUDIX hydrolase	-
SM_RS10485 (SMc04274)	2202294..2203400	-	1107	WP_010969661.1	endonuclease	-
SM_RS10490 (SMc04276)	2203551..2203994	-	444	WP_003529999.1	TIGR02594 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T20647 WP_003530010.1 NC_003047:2199475-2199663 [Sinorhizobium meliloti 1021]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T20647 NC_003047:2199475-2199663 [Sinorhizobium meliloti 1021]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT20647 WP_010969659.1 NC_003047:2199668-2200129 [Sinorhizobium meliloti 1021]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT20647 NC_003047:2199668-2200129 [Sinorhizobium meliloti 1021]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0