Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 509318..509954 | Replicon | chromosome |
| Accession | NZ_CP076731 | ||
| Organism | Bacillus subtilis subsp. subtilis strain Miz-8 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | KP952_RS02590 | Protein ID | WP_003156187.1 |
| Coordinates | 509604..509954 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | KP952_RS02585 | Protein ID | WP_003225183.1 |
| Coordinates | 509318..509599 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KP952_RS02565 (KP952_02565) | 505677..506276 | - | 600 | WP_003246687.1 | rhomboid family intramembrane serine protease | - |
| KP952_RS02570 (KP952_02570) | 506371..506736 | + | 366 | WP_014478897.1 | holo-ACP synthase | - |
| KP952_RS02575 (KP952_02575) | 506902..507918 | + | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| KP952_RS02580 (KP952_02580) | 508033..509202 | + | 1170 | WP_014478898.1 | alanine racemase | - |
| KP952_RS02585 (KP952_02585) | 509318..509599 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| KP952_RS02590 (KP952_02590) | 509604..509954 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KP952_RS02595 (KP952_02595) | 510070..510894 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| KP952_RS02600 (KP952_02600) | 510899..511264 | + | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| KP952_RS02605 (KP952_02605) | 511268..511669 | + | 402 | WP_014478899.1 | serine/threonine-protein kinase RsbT | - |
| KP952_RS02610 (KP952_02610) | 511681..512688 | + | 1008 | WP_014478900.1 | phosphoserine phosphatase RsbU | - |
| KP952_RS02615 (KP952_02615) | 512757..513086 | + | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| KP952_RS02620 (KP952_02620) | 513083..513565 | + | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| KP952_RS02625 (KP952_02625) | 513531..514319 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| KP952_RS02630 (KP952_02630) | 514319..514918 | + | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T206466 WP_003156187.1 NZ_CP076731:509604-509954 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T206466 NZ_CP102436:c62926-62615 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT206466 WP_003225183.1 NZ_CP076731:509318-509599 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT206466 NZ_CP102436:c62618-62199 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|