Detailed information of TA system TA206456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 300352..300954
• Replicon: chromosome
• Accession: NZ_CP076724
• Organism: Geomonas nitrogeniifigens strain RG29

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KP005_RS01305
• Protein ID: WP_216497472.1
• Coordinates: 300658..300954 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KP005_RS01300
• Protein ID: WP_216497459.1
• Coordinates: 300352..300654 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP005_RS01275 (KP005_01275)	295676..295870	+	195	WP_216500854.1	hypothetical protein	-
KP005_RS01280 (KP005_01280)	295911..297065	+	1155	WP_216790274.1	DUF4388 domain-containing protein	-
KP005_RS01285 (KP005_01285)	298261..298884	+	624	WP_216790276.1	hypothetical protein	-
KP005_RS01290 (KP005_01290)	298990..299466	+	477	WP_216497461.1	nuclear transport factor 2 family protein	-
KP005_RS01295 (KP005_01295)	299685..300158	+	474	WP_216497460.1	hypothetical protein	-
KP005_RS01300 (KP005_01300)	300352..300654	-	303	WP_216497459.1	putative addiction module antidote protein	Antitoxin
KP005_RS01305 (KP005_01305)	300658..300954	-	297	WP_216497472.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KP005_RS01310 (KP005_01310)	301088..301519	+	432	WP_216497458.1	hypothetical protein	-
KP005_RS01315 (KP005_01315)	301531..302061	+	531	WP_216790277.1	hypothetical protein	-
KP005_RS01320 (KP005_01320)	302177..302854	+	678	WP_236026719.1	hypothetical protein	-
KP005_RS01325 (KP005_01325)	303004..303345	+	342	WP_216497456.1	cupin domain-containing protein	-
KP005_RS01330 (KP005_01330)	303362..304060	+	699	WP_216790279.1	hypothetical protein	-
KP005_RS01335 (KP005_01335)	304205..304861	+	657	WP_236026718.1	DUF2459 domain-containing protein	-
KP005_RS01340 (KP005_01340)	304877..305497	-	621	WP_216790280.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	289264..303345	14081

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (10-96)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11161.89 Da
• Isoelectric Point: 9.9861

>T206456 WP_216497472.1 NZ_CP076724:c300954-300658 [Geomonas nitrogeniifigens]
MYIVKTLPEFDAWLNGIKDRTTRLRLARRLDKAQRGNLGDVKPVGHGVFEMREFFGSGWRMYYIERGNILIVMLGGGDKS
TQAADIAIAFERAARLED

Nucleotide

• Length: 297 bp

>T206456 NZ_CP102435:c938332-938114 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10704.45 Da
• Isoelectric Point: 7.3589

>AT206456 WP_216497459.1 NZ_CP076724:c300654-300352 [Geomonas nitrogeniifigens]
MPKKVVVSDLPEFDLAMQLKSEADIAAYITMVIEEGDAAELAHALGVAARARGMAEIAHAAGLTREALYKALRPGAHPRF
DTISRVCSALGVRLVAQPRH

Nucleotide

• Length: 303 bp

>AT206456 NZ_CP102435:c938733-938359 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG