Detailed information of TA system TA206445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 56418..57043
• Replicon: plasmid unnamed3
• Accession: NZ_CP076712
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KQ220_RS25175
• Protein ID: WP_000911317.1
• Coordinates: 56418..56816 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A828HMU3
• Locus tag: KQ220_RS25180
• Protein ID: WP_059320917.1
• Coordinates: 56816..57043 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS25155 (51970)	51970..52449	+	480	WP_059320959.1	surface exclusion protein	-
KQ220_RS25160 (52498)	52498..53229	+	732	WP_059320920.1	conjugal transfer complement resistance protein TraT	-
KQ220_RS25165 (53432)	53432..54169	+	738	WP_059320919.1	hypothetical protein	-
KQ220_RS25170 (54220)	54220..56409	+	2190	WP_216781588.1	type IV conjugative transfer system coupling protein TraD	-
KQ220_RS25175 (56418)	56418..56816	-	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
KQ220_RS25180 (56816)	56816..57043	-	228	WP_059320917.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..66958	66958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T206445 WP_000911317.1 NZ_CP076712:c56816-56418 [Escherichia coli O158:H23]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T206445 NZ_CP102396:1190-1456 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8583.63 Da
• Isoelectric Point: 4.7513

>AT206445 WP_059320917.1 NZ_CP076712:c57043-56816 [Escherichia coli O158:H23]
METTVFFSNRSQAVRLPKAVALPENIKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT206445 NZ_CP102396:982-1206 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A828HMU3