Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 53481..54016 Replicon plasmid unnamed2
Accession NZ_CP076711
Organism Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag KQ220_RS24660 Protein ID WP_000222766.1
Coordinates 53481..53768 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag KQ220_RS24665 Protein ID WP_001132900.1
Coordinates 53765..54016 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ220_RS24625 (48518) 48518..48778 + 261 WP_216781562.1 hypothetical protein -
KQ220_RS24630 (48775) 48775..49362 + 588 Protein_60 hypothetical protein -
KQ220_RS24635 (49384) 49384..49683 - 300 Protein_61 DUF6404 family protein -
KQ220_RS25480 (50200) 50200..52057 - 1858 Protein_62 colicin-like pore-forming protein -
KQ220_RS24655 (52907) 52907..53311 - 405 WP_000154256.1 DUF2251 domain-containing protein -
KQ220_RS24660 (53481) 53481..53768 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KQ220_RS24665 (53765) 53765..54016 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KQ220_RS24670 (54986) 54986..55843 - 858 WP_158709091.1 incFII family plasmid replication initiator RepA -
KQ220_RS25490 (55862) 55862..56038 - 177 Protein_67 protein CopA/IncA -
KQ220_RS24680 (56141) 56141..56398 - 258 WP_106906995.1 replication regulatory protein RepA -
KQ220_RS24685 (56682) 56682..56831 - 150 WP_106906997.1 Hok/Gef family protein -
- (56876) 56876..56935 + 60 NuclAT_0 - -
- (56876) 56876..56935 + 60 NuclAT_0 - -
- (56876) 56876..56935 + 60 NuclAT_0 - -
- (56876) 56876..56935 + 60 NuclAT_0 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..69043 69043


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T206437 WP_000222766.1 NZ_CP076711:c53768-53481 [Escherichia coli O158:H23]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T206437 NZ_CP102390:c4792647-4792363 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT206437 WP_001132900.1 NZ_CP076711:c54016-53765 [Escherichia coli O158:H23]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT206437 NZ_CP102390:c4792879-4792637 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References