Detailed information of TA system TA206436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 8070..8595
• Replicon: plasmid unnamed2
• Accession: NZ_CP076711
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: KQ220_RS24385
• Protein ID: WP_106907027.1
• Coordinates: 8070..8375 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: KQ220_RS24390
• Protein ID: WP_000813639.1
• Coordinates: 8377..8595 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS24355 (3169)	3169..3561	-	393	WP_000272016.1	plasmid partitioning/stability family protein	-
KQ220_RS24360 (3565)	3565..4539	-	975	WP_097469860.1	plasmid segregation protein ParM	-
KQ220_RS24365 (4779)	4779..5153	-	375	WP_106907023.1	hypothetical protein	-
KQ220_RS24370 (5153)	5153..5785	-	633	WP_000312330.1	ParA family protein	-
KQ220_RS24375 (6541)	6541..7296	-	756	WP_000807692.1	replication initiation protein RepE	-
KQ220_RS24380 (7799)	7799..8069	-	271	Protein_10	tyrosine-type recombinase/integrase	-
KQ220_RS24385 (8070)	8070..8375	-	306	WP_106907027.1	type II toxin-antitoxin system toxin CcdB	Toxin
KQ220_RS24390 (8377)	8377..8595	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KQ220_RS24395 (8988)	8988..9371	-	384	Protein_13	Tn3 family transposase	-
KQ220_RS24400 (9479)	9479..9853	-	375	Protein_14	transposase zinc-binding domain-containing protein	-
KQ220_RS24405 (9808)	9808..10056	-	249	WP_158709094.1	hypothetical protein	-
KQ220_RS24410 (10202)	10202..10458	+	257	Protein_16	IS3 family transposase	-
KQ220_RS24415 (10709)	10709..11575	-	867	WP_225932707.1	AraC family transcriptional regulator	-
KQ220_RS24420 (12591)	12591..12839	+	249	WP_216781571.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..69043	69043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11726.50 Da
• Isoelectric Point: 6.4674

>T206436 WP_106907027.1 NZ_CP076711:c8375-8070 [Escherichia coli O158:H23]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADFSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T206436 NZ_CP102390:c4690432-4689899 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT206436 WP_000813639.1 NZ_CP076711:c8595-8377 [Escherichia coli O158:H23]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT206436 NZ_CP102390:c4690709-4690443 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DIR5