Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4799643..4800238 Replicon chromosome
Accession NZ_CP076709
Organism Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)


Gene name chpB Uniprot ID V0SXT4
Locus tag KQ220_RS23000 Protein ID WP_000239581.1
Coordinates 4799888..4800238 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag KQ220_RS22995 Protein ID WP_001223213.1
Coordinates 4799643..4799894 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ220_RS22985 (4795308) 4795308..4799087 + 3780 WP_000060904.1 autotransporter assembly complex protein TamB -
KQ220_RS22990 (4799090) 4799090..4799431 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KQ220_RS22995 (4799643) 4799643..4799894 + 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KQ220_RS23000 (4799888) 4799888..4800238 + 351 WP_000239581.1 endoribonuclease toxin ChpB Toxin
KQ220_RS23005 (4800317) 4800317..4800847 - 531 WP_000055075.1 inorganic diphosphatase -
KQ220_RS23010 (4801157) 4801157..4802113 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KQ220_RS23015 (4802246) 4802246..4803748 + 1503 WP_085460167.1 sugar ABC transporter ATP-binding protein -
KQ220_RS23020 (4803762) 4803762..4804784 + 1023 WP_001305656.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12453.41 Da        Isoelectric Point: 6.2206

>T206435 WP_000239581.1 NZ_CP076709:4799888-4800238 [Escherichia coli O158:H23]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T206435 NZ_CP102390:4093862-4094080 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT206435 WP_001223213.1 NZ_CP076709:4799643-4799894 [Escherichia coli O158:H23]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT206435 NZ_CP102390:4093461-4093835 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SXT4

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References