Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3919454..3920085 Replicon chromosome
Accession NZ_CP076709
Organism Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag KQ220_RS18995 Protein ID WP_001260301.1
Coordinates 3919810..3920085 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KQ220_RS18990 Protein ID WP_000593555.1
Coordinates 3919454..3919813 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ220_RS18965 (3915586) 3915586..3916530 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
KQ220_RS18970 (3916527) 3916527..3917360 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KQ220_RS18975 (3917360) 3917360..3918124 + 765 WP_001136197.1 nickel import ATP-binding protein NikD -
KQ220_RS18980 (3918121) 3918121..3918927 + 807 WP_048971478.1 nickel import ATP-binding protein NikE -
KQ220_RS18985 (3918933) 3918933..3919334 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KQ220_RS18990 (3919454) 3919454..3919813 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KQ220_RS18995 (3919810) 3919810..3920085 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
KQ220_RS19000 (3920158) 3920158..3921282 - 1125 WP_001318088.1 ABC transporter permease -
KQ220_RS19005 (3921282) 3921282..3924017 - 2736 WP_059341956.1 ribosome-associated ATPase/putative transporter RbbA -
KQ220_RS19010 (3924014) 3924014..3925081 - 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T206433 WP_001260301.1 NZ_CP076709:c3920085-3919810 [Escherichia coli O158:H23]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T206433 NZ_CP102390:2012846-2012948 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT206433 WP_000593555.1 NZ_CP076709:c3919813-3919454 [Escherichia coli O158:H23]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT206433 NZ_CP102390:c2012955-2012810 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References