Detailed information of TA system TA206420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1727385..1728973
• Replicon: chromosome
• Accession: NZ_CP076709
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: KQ220_RS08175
• Protein ID: WP_001125415.1
• Coordinates: 1727385..1728707 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KQ220_RS08180
• Protein ID: WP_001296726.1
• Coordinates: 1728707..1728973 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS08140 (1723119)	1723119..1723658	-	540	Protein_1595	aldo/keto reductase	-
KQ220_RS08145 (1723661)	1723661..1724233	-	573	WP_112006032.1	NAD(P)H-dependent oxidoreductase	-
KQ220_RS25570 (1724329)	1724329..1724481	-	153	WP_001314732.1	amidohydrolase family protein	-
KQ220_RS08155 (1724471)	1724471..1724890	-	420	WP_001327999.1	amidohydrolase family protein	-
KQ220_RS08160 (1724850)	1724850..1725005	-	156	WP_001327998.1	hypothetical protein	-
KQ220_RS08165 (1725108)	1725108..1726055	-	948	WP_083575867.1	aldo/keto reductase	-
KQ220_RS08170 (1726083)	1726083..1726987	+	905	Protein_1601	LysR family transcriptional regulator	-
KQ220_RS08175 (1727385)	1727385..1728707	-	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KQ220_RS08180 (1728707)	1728707..1728973	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T206420 WP_001125415.1 NZ_CP076709:c1728707-1727385 [Escherichia coli O158:H23]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T206420 NZ_CP102380:4230300-4230473 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT206420 WP_001296726.1 NZ_CP076709:c1728973-1728707 [Escherichia coli O158:H23]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT206420 NZ_CP102380:4229976-4230272 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: U9YVT8