Detailed information of TA system TA206418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1629798..1630436
• Replicon: chromosome
• Accession: NZ_CP076709
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: KQ220_RS07730
• Protein ID: WP_000813795.1
• Coordinates: 1629798..1629974 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KQ220_RS07735
• Protein ID: WP_001270286.1
• Coordinates: 1630020..1630436 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS07710 (1625420)	1625420..1626592	-	1173	WP_024044351.1	BenE family transporter YdcO	-
KQ220_RS07715 (1626684)	1626684..1627220	+	537	WP_024044350.1	DNA-binding transcriptional regulator SutR	-
KQ220_RS07720 (1627293)	1627293..1629254	+	1962	WP_024216495.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KQ220_RS07725 (1629346)	1629346..1629576	-	231	WP_000494244.1	YncJ family protein	-
KQ220_RS07730 (1629798)	1629798..1629974	+	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KQ220_RS07735 (1630020)	1630020..1630436	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KQ220_RS07740 (1630515)	1630515..1631921	+	1407	WP_024042139.1	PLP-dependent aminotransferase family protein	-
KQ220_RS07745 (1632166)	1632166..1633311	+	1146	WP_024042138.1	ABC transporter substrate-binding protein	-
KQ220_RS07750 (1633329)	1633329..1634342	+	1014	WP_024042137.1	ABC transporter ATP-binding protein	-
KQ220_RS07755 (1634343)	1634343..1635284	+	942	WP_024216494.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T206418 WP_000813795.1 NZ_CP076709:1629798-1629974 [Escherichia coli O158:H23]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T206418 NZ_CP102380:c3105558-3105244 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT206418 WP_001270286.1 NZ_CP076709:1630020-1630436 [Escherichia coli O158:H23]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT206418 NZ_CP102380:c3105247-3104831 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1