Detailed information of TA system TA206412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 104008..104567
• Replicon: plasmid unnamed1
• Accession: NZ_CP076707
• Organism: Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KQ217_RS24200
• Protein ID: WP_216803922.1
• Coordinates: 104008..104283 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KQ217_RS24205
• Protein ID: WP_216803923.1
• Coordinates: 104283..104567 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ217_RS24170 (100032)	100032..100849	+	818	Protein_99	transposase	-
KQ217_RS24175 (101073)	101073..102194	-	1122	Protein_100	AAA family ATPase	-
KQ217_RS24180 (102269)	102269..102578	-	310	Protein_101	VapC toxin family PIN domain ribonuclease	-
KQ217_RS24185 (102575)	102575..102694	-	120	Protein_102	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
KQ217_RS24190 (102697)	102697..103659	+	963	Protein_103	relaxase domain-containing protein	-
KQ217_RS24195 (103657)	103657..103830	-	174	Protein_104	helix-turn-helix domain-containing protein	-
KQ217_RS24200 (104008)	104008..104283	-	276	WP_216803922.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQ217_RS24205 (104283)	104283..104567	-	285	WP_216803923.1	ribbon-helix-helix protein, CopG family	Antitoxin
KQ217_RS24210 (105457)	105457..105828	+	372	WP_225915880.1	DUF305 domain-containing protein	-
KQ217_RS24215 (105815)	105815..106879	+	1065	WP_216803925.1	YncE family protein	-
KQ217_RS24220 (107100)	107100..107249	+	150	Protein_109	accessory colonization factor AcfC	-
KQ217_RS24225 (108131)	108131..108586	-	456	Protein_110	plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	phzB1 / clbA / stcE / exeG / afaC-VII / papI / papB / papA / papH / papC / papD / papJ / papK / papE / papF / iutA / iucD / iucC / iucB / iucA / pic	1..128277	128277
	-	flank	IS/Tn	-	-	100077..100406	329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10764.50 Da
• Isoelectric Point: 9.9324

>T206412 WP_216803922.1 NZ_CP076707:c104283-104008 [Escherichia coli O170:H18]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFVGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T206412 NZ_CP102380:c1968061-1967810 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10446.88 Da
• Isoelectric Point: 5.6558

>AT206412 WP_216803923.1 NZ_CP076707:c104567-104283 [Escherichia coli O170:H18]
MQMKNNSAQATKVITAHVPLLMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT206412 NZ_CP102380:c1967813-1967559 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA