Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4048865..4049483 Replicon chromosome
Accession NZ_CP076706
Organism Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KQ217_RS19575 Protein ID WP_001291435.1
Coordinates 4048865..4049083 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KQ217_RS19580 Protein ID WP_000344800.1
Coordinates 4049109..4049483 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ217_RS19540 (4044153) 4044153..4044725 + 573 WP_000779831.1 YbaY family lipoprotein -
KQ217_RS19545 (4044756) 4044756..4045067 - 312 WP_000409908.1 MGMT family protein -
KQ217_RS19555 (4045446) 4045446..4045799 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KQ217_RS19560 (4045841) 4045841..4047391 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KQ217_RS19565 (4047555) 4047555..4048025 - 471 WP_000136192.1 YlaC family protein -
KQ217_RS19570 (4048141) 4048141..4048692 - 552 WP_000102539.1 maltose O-acetyltransferase -
KQ217_RS19575 (4048865) 4048865..4049083 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KQ217_RS19580 (4049109) 4049109..4049483 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KQ217_RS19585 (4050028) 4050028..4053177 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
KQ217_RS19590 (4053200) 4053200..4054393 - 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T206406 WP_001291435.1 NZ_CP076706:c4049083-4048865 [Escherichia coli O170:H18]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T206406 NZ_CP102380:1381725-1381901 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT206406 WP_000344800.1 NZ_CP076706:c4049483-4049109 [Escherichia coli O170:H18]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT206406 NZ_CP102380:1381947-1382363 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References