Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3557338..3557888 Replicon chromosome
Accession NZ_CP076706
Organism Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag KQ217_RS17250 Protein ID WP_001160966.1
Coordinates 3557574..3557888 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KQ217_RS17245 Protein ID WP_000125566.1
Coordinates 3557338..3557571 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ217_RS17220 (3552411) 3552411..3552698 + 288 WP_000203741.1 ferredoxin-like protein FixX -
KQ217_RS17225 (3552757) 3552757..3554088 + 1332 WP_001183207.1 MFS transporter -
KQ217_RS17230 (3554196) 3554196..3554726 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KQ217_RS17235 (3554719) 3554719..3556581 + 1863 WP_216803529.1 glutathione-regulated potassium-efflux system protein KefC -
KQ217_RS17240 (3556773) 3556773..3557252 + 480 WP_097246653.1 type 3 dihydrofolate reductase -
KQ217_RS17245 (3557338) 3557338..3557571 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KQ217_RS17250 (3557574) 3557574..3557888 + 315 WP_001160966.1 CcdB family protein Toxin
KQ217_RS17255 (3557885) 3557885..3558733 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KQ217_RS17260 (3558740) 3558740..3559117 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KQ217_RS17265 (3559120) 3559120..3559941 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KQ217_RS17270 (3559938) 3559938..3560927 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KQ217_RS17275 (3560927) 3560927..3562213 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T206404 WP_001160966.1 NZ_CP076706:3557574-3557888 [Escherichia coli O170:H18]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T206404 NZ_CP102380:c1143983-1143876 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT206404 WP_000125566.1 NZ_CP076706:3557338-3557571 [Escherichia coli O170:H18]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT206404 NZ_CP102380:1144030-1144096 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References