Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3179802..3180299 Replicon chromosome
Accession NZ_CP076706
Organism Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KQ217_RS15350 Protein ID WP_001173343.1
Coordinates 3180126..3180299 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag KQ217_RS15345 Protein ID WP_001312331.1
Coordinates 3179802..3180098 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ217_RS15325 (3176532) 3176532..3177251 - 720 WP_000611291.1 two-component system response regulator DcuR -
KQ217_RS15330 (3177248) 3177248..3178879 - 1632 WP_001216467.1 sensor histidine kinase -
KQ217_RS15335 (3179060) 3179060..3179290 + 231 WP_000373292.1 4Fe-4S mono-cluster protein YjdI -
KQ217_RS15340 (3179302) 3179302..3179574 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
KQ217_RS15345 (3179802) 3179802..3180098 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KQ217_RS15350 (3180126) 3180126..3180299 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KQ217_RS15355 (3180418) 3180418..3181935 - 1518 WP_000003805.1 lysine--tRNA ligase -
KQ217_RS15360 (3182172) 3182172..3183629 - 1458 WP_110140570.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T206396 WP_001173343.1 NZ_CP076706:3180126-3180299 [Escherichia coli O170:H18]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T206396 NZ_CP102379:4229161-4229334 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT206396 WP_001312331.1 NZ_CP076706:3179802-3180098 [Escherichia coli O170:H18]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT206396 NZ_CP102379:4228837-4229133 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References