Detailed information of TA system TA206375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 64252..64877
• Replicon: plasmid unnamed
• Accession: NZ_CP076705
• Organism: Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KQ221_RS23420
• Protein ID: WP_042966721.1
• Coordinates: 64479..64877 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: KQ221_RS23415
• Protein ID: WP_000450526.1
• Coordinates: 64252..64479 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ221_RS23415 (64252)	64252..64479	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KQ221_RS23420 (64479)	64479..64877	+	399	WP_042966721.1	type II toxin-antitoxin system VapC family toxin	Toxin
KQ221_RS23425 (64886)	64886..67102	-	2217	WP_096263336.1	type IV conjugative transfer system coupling protein TraD	-
KQ221_RS23430 (67384)	67384..67995	-	612	WP_000908442.1	hypothetical protein	-
KQ221_RS23435 (68067)	68067..68777	-	711	WP_052588736.1	conjugal transfer complement resistance protein TraT	-
KQ221_RS23440 (68830)	68830..69327	-	498	WP_032211977.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..117710	117710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.21 Da
• Isoelectric Point: 7.8605

>T206375 WP_042966721.1 NZ_CP076705:64479-64877 [Escherichia coli O8:H28]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRTELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T206375 NZ_CP102378:c4590476-4590135 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT206375 WP_000450526.1 NZ_CP076705:64252-64479 [Escherichia coli O8:H28]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT206375 NZ_CP102378:4590471-4590547 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT