Detailed information of TA system TA206366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4204169..4204691
• Replicon: chromosome
• Accession: NZ_CP076704
• Organism: Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KQ221_RS20380
• Protein ID: WP_001105431.1
• Coordinates: 4204169..4204459 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SC69
• Locus tag: KQ221_RS20385
• Protein ID: WP_000212715.1
• Coordinates: 4204449..4204691 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ221_RS20365 (4199332)	4199332..4200987	+	1656	WP_001307546.1	alpha,alpha-phosphotrehalase	-
KQ221_RS20370 (4201381)	4201381..4203519	+	2139	WP_000187778.1	anaerobic ribonucleoside-triphosphate reductase	-
KQ221_RS20375 (4203704)	4203704..4204168	+	465	WP_001009181.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
KQ221_RS20380 (4204169)	4204169..4204459	-	291	WP_001105431.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQ221_RS20385 (4204449)	4204449..4204691	-	243	WP_000212715.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KQ221_RS20390 (4204883)	4204883..4205269	-	387	WP_001232240.1	cytochrome b562	-
KQ221_RS20395 (4205452)	4205452..4206804	-	1353	WP_001162162.1	metalloprotease PmbA	-
KQ221_RS20400 (4206898)	4206898..4207449	+	552	WP_000166270.1	ribosome biogenesis factor YjgA	-
KQ221_RS20405 (4207600)	4207600..4208973	-	1374	WP_001219792.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11368.26 Da
• Isoelectric Point: 10.0238

>T206366 WP_001105431.1 NZ_CP076704:c4204459-4204169 [Escherichia coli O8:H28]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTLGYRLVYQVNDKELLVLVISIGK
RENSAVYEDATKRLDE

Nucleotide

• Length: 291 bp

>T206366 NZ_CP102378:c2921727-2921392 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9125.56 Da
• Isoelectric Point: 5.1369

>AT206366 WP_000212715.1 NZ_CP076704:c4204691-4204449 [Escherichia coli O8:H28]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT206366 NZ_CP102378:c2921975-2921727 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9H4B3