Detailed information of TA system TA206365

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4147835..4148538
• Replicon: chromosome
• Accession: NZ_CP076704
• Organism: Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A853JWF2
• Locus tag: KQ221_RS20100
• Protein ID: WP_032267594.1
• Coordinates: 4147835..4148176 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: KQ221_RS20105
• Protein ID: WP_032267593.1
• Coordinates: 4148197..4148538 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ221_RS20075 (4142945)	4142945..4143925	+	981	WP_001366032.1	sialate O-acetylesterase	-
KQ221_RS20080 (4143933)	4143933..4144037	-	105	Protein_3930	HNH endonuclease	-
KQ221_RS20085 (4144137)	4144137..4145009	+	873	WP_000168567.1	HNH endonuclease	-
KQ221_RS20090 (4145120)	4145120..4146118	-	999	WP_001240355.1	membrane protein	-
KQ221_RS20095 (4146577)	4146577..4147584	-	1008	WP_032267595.1	restriction endonuclease	-
KQ221_RS20100 (4147835)	4147835..4148176	-	342	WP_032267594.1	TA system toxin CbtA family protein	Toxin
KQ221_RS20105 (4148197)	4148197..4148538	-	342	WP_032267593.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KQ221_RS20110 (4148549)	4148549..4149091	-	543	WP_032267592.1	DNA repair protein RadC	-
KQ221_RS20115 (4149104)	4149104..4149547	-	444	WP_032267591.1	antirestriction protein	-
KQ221_RS20120 (4149578)	4149578..4150399	-	822	WP_032267590.1	DUF932 domain-containing protein	-
KQ221_RS20125 (4150520)	4150520..4150993	-	474	WP_032279719.1	hypothetical protein	-
KQ221_RS20130 (4151088)	4151088..4151627	-	540	WP_032279718.1	DUF4339 domain-containing protein	-
KQ221_RS23920 (4151694)	4151694..4151981	-	288	WP_235839820.1	hypothetical protein	-
KQ221_RS20140 (4152151)	4152151..4152606	-	456	WP_057688286.1	hypothetical protein	-
KQ221_RS20145 (4152645)	4152645..4153280	-	636	WP_032267586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4137907..4170870	32963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12767.68 Da
• Isoelectric Point: 6.8699

>T206365 WP_032267594.1 NZ_CP076704:c4148176-4147835 [Escherichia coli O8:H28]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDEKVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
GWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T206365 NZ_CP102378:2788089-2788418 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12760.46 Da
• Isoelectric Point: 6.6213

>AT206365 WP_032267593.1 NZ_CP076704:c4148538-4148197 [Escherichia coli O8:H28]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDLAFPHFISQMESMLTTGEINPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYLTYR

Nucleotide

• Length: 342 bp

>AT206365 NZ_CP102378:2787751-2788068 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A853JWF2