Detailed information of TA system TA206363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3506432..3507269
• Replicon: chromosome
• Accession: NZ_CP076704
• Organism: Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KQ221_RS17020
• Protein ID: WP_000227784.1
• Coordinates: 3506727..3507269 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KQ221_RS17015
• Protein ID: WP_001297137.1
• Coordinates: 3506432..3506743 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ221_RS16990 (3501452)	3501452..3502399	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KQ221_RS16995 (3502421)	3502421..3504412	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KQ221_RS17000 (3504402)	3504402..3505016	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KQ221_RS17005 (3505016)	3505016..3505345	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KQ221_RS17010 (3505357)	3505357..3506247	+	891	WP_000971336.1	heme o synthase	-
KQ221_RS17015 (3506432)	3506432..3506743	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KQ221_RS17020 (3506727)	3506727..3507269	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KQ221_RS17025 (3507325)	3507325..3508260	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
KQ221_RS17030 (3508668)	3508668..3510032	+	1365	WP_001000975.1	MFS transporter	-
KQ221_RS17035 (3510160)	3510160..3510651	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KQ221_RS17040 (3510819)	3510819..3511730	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T206363 WP_000227784.1 NZ_CP076704:3506727-3507269 [Escherichia coli O8:H28]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T206363 NZ_CP102378:c2100349-2100098 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT206363 WP_001297137.1 NZ_CP076704:3506432-3506743 [Escherichia coli O8:H28]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT206363 NZ_CP102378:c2100101-2099847 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3