Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3472343..3472961 Replicon chromosome
Accession NZ_CP076704
Organism Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KQ221_RS16850 Protein ID WP_001291435.1
Coordinates 3472743..3472961 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KQ221_RS16845 Protein ID WP_000344800.1
Coordinates 3472343..3472717 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ221_RS16835 (3467432) 3467432..3468625 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KQ221_RS16840 (3468648) 3468648..3471797 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KQ221_RS16845 (3472343) 3472343..3472717 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KQ221_RS16850 (3472743) 3472743..3472961 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KQ221_RS16855 (3473132) 3473132..3473683 + 552 WP_000102578.1 maltose O-acetyltransferase -
KQ221_RS16860 (3473799) 3473799..3474269 + 471 WP_000136192.1 YlaC family protein -
KQ221_RS16865 (3474433) 3474433..3475983 + 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KQ221_RS16870 (3476025) 3476025..3476378 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KQ221_RS16880 (3476757) 3476757..3477068 + 312 WP_000409911.1 MGMT family protein -
KQ221_RS16885 (3477099) 3477099..3477671 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T206362 WP_001291435.1 NZ_CP076704:3472743-3472961 [Escherichia coli O8:H28]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T206362 NZ_CP102378:c2100101-2099847 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT206362 WP_000344800.1 NZ_CP076704:3472343-3472717 [Escherichia coli O8:H28]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT206362 NZ_CP102378:c2100349-2100098 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References