Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1338760..1339385 Replicon chromosome
Accession NZ_CP076704
Organism Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag KQ221_RS06530 Protein ID WP_000911330.1
Coordinates 1338987..1339385 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KQ221_RS06525 Protein ID WP_000450524.1
Coordinates 1338760..1338987 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ221_RS06500 (1334563) 1334563..1335033 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KQ221_RS06505 (1335033) 1335033..1335605 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KQ221_RS06510 (1335751) 1335751..1336629 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
KQ221_RS06515 (1336646) 1336646..1337680 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KQ221_RS06520 (1337892) 1337892..1338605 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KQ221_RS06525 (1338760) 1338760..1338987 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KQ221_RS06530 (1338987) 1338987..1339385 + 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
KQ221_RS06535 (1339532) 1339532..1340395 + 864 WP_001267508.1 neutral zinc metallopeptidase -
KQ221_RS06540 (1340410) 1340410..1342425 + 2016 WP_000829355.1 tRNA cytosine(34) acetyltransferase TmcA -
KQ221_RS06545 (1342499) 1342499..1343197 + 699 WP_000679823.1 esterase -
KQ221_RS06550 (1343307) 1343307..1343507 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T206351 WP_000911330.1 NZ_CP076704:1338987-1339385 [Escherichia coli O8:H28]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T206351 NZ_CP102378:c263669-263328 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT206351 WP_000450524.1 NZ_CP076704:1338760-1338987 [Escherichia coli O8:H28]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT206351 NZ_CP102378:c264007-263690 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References