Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 941777..942462 Replicon chromosome
Accession NZ_CP076704
Organism Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)


Gene name ypjF Uniprot ID -
Locus tag KQ221_RS04600 Protein ID WP_097446464.1
Coordinates 942154..942462 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID -
Locus tag KQ221_RS04595 Protein ID WP_097446463.1
Coordinates 941777..942133 (+) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ221_RS04550 (937074) 937074..937778 + 705 WP_097446457.1 WYL domain-containing protein -
KQ221_RS04555 (937778) 937778..938347 + 570 WP_097446458.1 hypothetical protein -
KQ221_RS04560 (938384) 938384..938836 + 453 WP_000737933.1 hypothetical protein -
KQ221_RS04565 (938833) 938833..939282 + 450 WP_015699704.1 IrmA family protein -
KQ221_RS04570 (939354) 939354..939584 + 231 WP_096836431.1 DUF905 domain-containing protein -
KQ221_RS04575 (939683) 939683..940504 + 822 WP_097446459.1 DUF932 domain-containing protein -
KQ221_RS04580 (940535) 940535..940978 + 444 WP_097446460.1 antirestriction protein -
KQ221_RS04585 (940991) 940991..941533 + 543 WP_097446461.1 DNA repair protein RadC -
KQ221_RS04590 (941530) 941530..941751 + 222 WP_097446462.1 DUF987 family protein -
KQ221_RS04595 (941777) 941777..942133 + 357 WP_097446463.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KQ221_RS04600 (942154) 942154..942462 + 309 WP_097446464.1 TA system toxin CbtA family protein Toxin
KQ221_RS04605 (942918) 942918..944528 + 1611 WP_123006992.1 glycoside hydrolase family 15 protein -
KQ221_RS04610 (944541) 944541..944660 + 120 Protein_906 type III secretion protein -
KQ221_RS04615 (944620) 944620..945607 + 988 Protein_907 YscQ/HrcQ family type III secretion apparatus protein -
KQ221_RS04620 (945597) 945597..946262 + 666 WP_000071534.1 EscR/YscR/HrcR family type III secretion system export apparatus protein -
KQ221_RS04625 (946272) 946272..946531 + 260 Protein_909 EscS/YscS/HrcS family type III secretion system export apparatus protein -
KQ221_RS04630 (946533) 946533..947300 + 768 WP_000503287.1 type III secretion system export apparatus subunit SctT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - spaP / spaQ 930581..961083 30502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-97)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11826.79 Da        Isoelectric Point: 5.6466

>T206349 WP_097446464.1 NZ_CP076704:942154-942462 [Escherichia coli O8:H28]
MKFLPTTNMRAARPCLSSVAVWQMLLTRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRAVAIWV

Download         Length: 309 bp

>T206349 NZ_CP102378:c246239-245961 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13101.83 Da        Isoelectric Point: 5.4702

>AT206349 WP_097446463.1 NZ_CP076704:941777-942133 [Escherichia coli O8:H28]
MNNHSESGAIPENPACQQWGLKRAMTPCFGARLVQEGDRLHFLADRAGFNGAFCDDDALYLDQAFPLILKQLELILTSGE
LSPRHQHCVTLYHNGLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 357 bp

>AT206349 NZ_CP102378:c246502-246242 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References