Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 855959..856613 Replicon chromosome
Accession NZ_CP076704
Organism Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KQ221_RS04185 Protein ID WP_000244777.1
Coordinates 856206..856613 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KQ221_RS04180 Protein ID WP_000354046.1
Coordinates 855959..856225 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ221_RS04155 (851128) 851128..851871 + 744 WP_097446517.1 SDR family oxidoreductase -
KQ221_RS04160 (851928) 851928..853361 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KQ221_RS04165 (853406) 853406..853717 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KQ221_RS04170 (853881) 853881..854540 + 660 WP_000250274.1 hemolysin III family protein -
KQ221_RS04175 (854736) 854736..855716 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KQ221_RS04180 (855959) 855959..856225 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KQ221_RS04185 (856206) 856206..856613 + 408 WP_000244777.1 protein YgfX Toxin
KQ221_RS04190 (856653) 856653..857174 - 522 WP_001055870.1 flavodoxin FldB -
KQ221_RS04195 (857286) 857286..858182 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KQ221_RS04200 (858207) 858207..858917 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KQ221_RS04205 (858923) 858923..860656 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T206348 WP_000244777.1 NZ_CP076704:856206-856613 [Escherichia coli O8:H28]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T206348 NZ_CP102374:c6780254-6779904 [Pseudomonas sp. T8]
ATGGGCGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAATTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGAGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGACGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTGAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT206348 WP_000354046.1 NZ_CP076704:855959-856225 [Escherichia coli O8:H28]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT206348 NZ_CP102374:c6779914-6779600 [Pseudomonas sp. T8]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTGCGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCACCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACCACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCACTGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References