Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 290419..291219 Replicon chromosome
Accession NZ_CP076704
Organism Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KQ221_RS01340 Protein ID WP_000342449.1
Coordinates 290692..291219 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KQ221_RS01335 Protein ID WP_001277108.1
Coordinates 290419..290685 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ221_RS01315 (286077) 286077..286745 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KQ221_RS01320 (286738) 286738..287796 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KQ221_RS01325 (288041) 288041..288895 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KQ221_RS01330 (289166) 289166..290269 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KQ221_RS01335 (290419) 290419..290685 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KQ221_RS01340 (290692) 290692..291219 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KQ221_RS01345 (291216) 291216..291599 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
KQ221_RS01350 (292023) 292023..293132 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KQ221_RS01355 (293180) 293180..294106 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KQ221_RS01360 (294103) 294103..295380 + 1278 WP_000803784.1 branched chain amino acid ABC transporter permease LivM -
KQ221_RS01365 (295377) 295377..296144 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T206346 WP_000342449.1 NZ_CP076704:290692-291219 [Escherichia coli O8:H28]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T206346 NZ_CP102374:5064064-5064363 [Pseudomonas sp. T8]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAATGGCCTTGCCGGTGACGTGCCACCGGTGGGCGAGGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGAGTTTATTTCCAGCAGCTTGGTTCGCAACTTGTCATTTTGTTATGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT206346 WP_001277108.1 NZ_CP076704:290419-290685 [Escherichia coli O8:H28]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT206346 NZ_CP102374:5064360-5064653 [Pseudomonas sp. T8]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAAGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCGTTCAGTGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGCGCCCTGGGGCTGGAGATGACGGCGCGGGTGCATGGGGCGTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References