Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 269130..269716 Replicon chromosome
Accession NZ_CP076704
Organism Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KQ221_RS01215 Protein ID WP_042014162.1
Coordinates 269130..269360 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KQ221_RS01220 Protein ID WP_000593555.1
Coordinates 269357..269716 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ221_RS01205 (265167) 265167..267902 + 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -
KQ221_RS01210 (267902) 267902..269026 + 1125 WP_001314210.1 ABC transporter permease -
KQ221_RS01215 (269130) 269130..269360 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KQ221_RS01220 (269357) 269357..269716 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KQ221_RS01225 (269836) 269836..270237 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KQ221_RS01230 (270242) 270242..271048 - 807 WP_097446330.1 nickel import ATP-binding protein NikE -
KQ221_RS01235 (271045) 271045..271809 - 765 WP_097446329.1 nickel import ATP-binding protein NikD -
KQ221_RS01240 (271809) 271809..272642 - 834 WP_097446328.1 nickel ABC transporter permease subunit NikC -
KQ221_RS01245 (272639) 272639..273583 - 945 WP_088144577.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T206345 WP_042014162.1 NZ_CP076704:269130-269360 [Escherichia coli O8:H28]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T206345 NZ_CP102374:c4078862-4078581 [Pseudomonas sp. T8]
ATGACTTATAAGCTCCAGTTTCTGCCTTCGGCGCGCAAGGAATGGGACAAGCTCGGTCATACCCTGCGCGAGCAATTCAA
GAAGAAGCTGGCCGAGCGCCTGGAAATGCCGCGGGTGCCCGCGGACGCCCTTCACGGTATGGCGGACTGCTACAAGATCA
AGCTCAAGGCCTCTGGCTATCGCCTGGTTTACCAAGTGATCGAGGATCAGGTCGTGGTTTCGGTGGTGGCCGTGGGCAAA
CGTGAATGCAGTGCCGTGTATGAGCGGGCACGAAAGCGCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT206345 WP_000593555.1 NZ_CP076704:269357-269716 [Escherichia coli O8:H28]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT206345 NZ_CP102374:c4079097-4078852 [Pseudomonas sp. T8]
ATGCAAAGCGTATTGGCTGATAAAGCTGTCAGCGTGTCTGAACTGAAAAAAAATCCTTCGGCTGTCCTGAACGGCGCACA
GGGTGGTGCGGTTGCGGTGCTCAATCACAACCGGGTCATGGGCTATATGGTCCCGGCCGACGTGTTTGAAGCCATGATGG
AGCGTCTTGACGATCTTGAACTGGCGGAGATTGTCCGTGCCCGTCGTCACGAGACCCCGGTCCCGGTAAATCTGGATGAC
TTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References