Detailed information of TA system TA206341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 68092..68651
• Replicon: plasmid pS10EC_A
• Accession: NZ_CP076699
• Organism: Escherichia coli strain S10EC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: KQ259_RS25115
• Protein ID: WP_000421272.1
• Coordinates: 68092..68367 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: KQ259_RS25120
• Protein ID: WP_001387467.1
• Coordinates: 68367..68651 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ259_RS25085 (KQ259_25085)	63266..64288	+	1023	WP_000361402.1	helicase UvrD	-
KQ259_RS25610	65273..65541	-	269	Protein_74	type II toxin-antitoxin system RelE/ParE family toxin	-
KQ259_RS25095 (KQ259_25095)	65528..65796	-	269	Protein_75	type II toxin-antitoxin system ParD family antitoxin	-
KQ259_RS25100 (KQ259_25100)	66328..67497	+	1170	WP_001072355.1	hypothetical protein	-
KQ259_RS25105 (KQ259_25105)	67530..67658	+	129	Protein_77	IS481 family transposase	-
KQ259_RS25110 (KQ259_25110)	67783..67986	-	204	Protein_78	hypothetical protein	-
KQ259_RS25115 (KQ259_25115)	68092..68367	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQ259_RS25120 (KQ259_25120)	68367..68651	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
KQ259_RS25125 (KQ259_25125)	69256..70008	+	753	WP_000562172.1	IclR family transcriptional regulator	-
KQ259_RS25130 (KQ259_25130)	70054..71019	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
KQ259_RS25135 (KQ259_25135)	71052..71432	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
KQ259_RS25140 (KQ259_25140)	71457..72347	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
KQ259_RS25145 (KQ259_25145)	72748..73188	+	441	Protein_85	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / mph(A) / sul1 / qacE / aadA5	-	1..102381	102381
	-	flank	IS/Tn	-	-	64542..65045	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T206341 WP_000421272.1 NZ_CP076699:c68367-68092 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T206341 NZ_CP102374:2751028-2751327 [Pseudomonas sp. T8]
ATGCACGTTGAGTGGCTGCGCAGCGCCCTCAAGAACCTCGATGACGAAGCCGCCTACATTGCACAGGACAGTCCTCAGGC
CGCCGCGGAATTCGTCAAAGCCATCCTGAGTAGCATTCAACACCTGAGCCGTTTTCCTGCCAGTGGTCGCGAAGGTCGCC
TGCCCGGTACCCGGGAGTGGGTGGTCCCCAATCGTCCTTACCTGATCCCCTACCGCGTACGTCAGGGACGCCTGCAGATA
CTGCGGATTTTCCACACCCGGCGCTTGCCGCCTGCCGGTTGGCAAGACGACCGCCAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT206341 WP_001387467.1 NZ_CP076699:c68651-68367 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT206341 NZ_CP102374:2750799-2751038 [Pseudomonas sp. T8]
ATGTCCGTCATGTCCTTGCGCCTGCCCGATGATGTTGCCGATACCCTGGCTCACTTGGCCAAGGCGACAGGCCGCAGCAA
GTCATTCCTCGCTGTGGATGCACTGAGAGAATATCTGGCCCGCGAAGCCTGGCAAATCAACGAGATCCAGAAAGCCCTGA
CTGAAGCAGAGGCAGGCGACTTCGCCAGCGCCGAAGAGCTGGATGGCGTACTCAACAAATGGACTGGCAATGCACGTTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880