206340

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60998..61641 Replicon plasmid pS10EC_A
Accession NZ_CP076699
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KQ259_RS25075 Protein ID WP_001034044.1
Coordinates 61225..61641 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KQ259_RS25070 Protein ID WP_001261286.1
Coordinates 60998..61228 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS25055 (KQ259_25055) 56135..56365 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
KQ259_RS25060 (KQ259_25060) 56362..56778 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KQ259_RS25065 (KQ259_25065) 56823..60617 - 3795 WP_001144732.1 hypothetical protein -
KQ259_RS25070 (KQ259_25070) 60998..61228 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KQ259_RS25075 (KQ259_25075) 61225..61641 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KQ259_RS25080 (KQ259_25080) 61716..63281 + 1566 WP_001128474.1 AAA family ATPase -
KQ259_RS25085 (KQ259_25085) 63266..64288 + 1023 WP_000361402.1 helicase UvrD -
KQ259_RS25610 65273..65541 - 269 Protein_74 type II toxin-antitoxin system RelE/ParE family toxin -
KQ259_RS25095 (KQ259_25095) 65528..65796 - 269 Protein_75 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / mph(A) / sul1 / qacE / aadA5 - 1..102381 102381
- flank IS/Tn - - 64542..65045 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T206340 WP_001034044.1 NZ_CP076699:61225-61641 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T206340 NZ_CP102374:1894595-1894777 [Pseudomonas sp. T8]
GTGCAAAGCAGACAGTTGATCAAGGAGCTTGAAGCCGCAGGCTGGGTGCTGGAGCGCGTTACCGGCAGCCATCATCTGTT
CAAGCATCCCTACCGGCCCGAGACCGTTCCGGTGCCTCACCCGAGGAAGGATCTGCCACGGGGGACGGTCAGGGCCATCC
AGAAACTGGCCGGGCTGATATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT206340 WP_001261286.1 NZ_CP076699:60998-61228 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT206340 NZ_CP102374:1894813-1895214 [Pseudomonas sp. T8]
GTGCACTATCCCATCTGCATCGAGTGGGGCGACGAGCACACTGCTACCGGCATCCAGATTCCTGACATTCCTGGCGCGGT
CACCGCCGGCGACACCTTCGAGGCCGCGTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGTG
AGGGACAAGCCATTCCCCTGCCCTCGCCCACCGGCACACACCGCGCCAACCCCGACTTCGAGGGGATGGGCTGGGGCATG
CTGGATATCGACATCACGCCTTACCTGGGCAAAACCGAAAAGGTCAATGTGACCCTGCCGGGCTACGTGATTCAACGAAT
CGACCGCTTTGTGCGGGAGCACAACATCAAGAGCCGGTCGTCTTTTCTGGCAGATGCGGCGATGGAAAAGCTGGGGCGTT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References