206339

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 56135..56778 Replicon plasmid pS10EC_A
Accession NZ_CP076699
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KQ259_RS25060 Protein ID WP_001034046.1
Coordinates 56362..56778 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KQ259_RS25055 Protein ID WP_001261278.1
Coordinates 56135..56365 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS25030 (KQ259_25030) 51957..52382 + 426 WP_000422741.1 transposase -
KQ259_RS25035 (KQ259_25035) 52379..52729 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
KQ259_RS25040 (KQ259_25040) 52760..54373 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
KQ259_RS25045 (KQ259_25045) 54584..55357 - 774 WP_000905949.1 hypothetical protein -
KQ259_RS25050 (KQ259_25050) 55370..55870 - 501 WP_000528932.1 HEPN family nuclease -
KQ259_RS25055 (KQ259_25055) 56135..56365 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KQ259_RS25060 (KQ259_25060) 56362..56778 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KQ259_RS25065 (KQ259_25065) 56823..60617 - 3795 WP_001144732.1 hypothetical protein -
KQ259_RS25070 (KQ259_25070) 60998..61228 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KQ259_RS25075 (KQ259_25075) 61225..61641 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / mph(A) / sul1 / qacE / aadA5 - 1..102381 102381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T206339 WP_001034046.1 NZ_CP076699:56362-56778 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T206339 NZ_CP102374:1867291-1867581 [Pseudomonas sp. T8]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT206339 WP_001261278.1 NZ_CP076699:56135-56365 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT206339 NZ_CP102374:1866996-1867289 [Pseudomonas sp. T8]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAACACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References