206338

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 49130..49655 Replicon plasmid pS10EC_A
Accession NZ_CP076699
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag KQ259_RS25010 Protein ID WP_001159868.1
Coordinates 49130..49435 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KQ259_RS25015 Protein ID WP_000813634.1
Coordinates 49437..49655 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS24995 (KQ259_24995) 45040..46206 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
KQ259_RS25000 (KQ259_25000) 46794..47549 - 756 WP_000852146.1 replication initiation protein RepE -
KQ259_RS25005 (KQ259_25005) 48323..49129 - 807 WP_000016982.1 site-specific integrase -
KQ259_RS25010 (KQ259_25010) 49130..49435 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
KQ259_RS25015 (KQ259_25015) 49437..49655 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KQ259_RS25020 (KQ259_25020) 50201..50713 + 513 WP_000151784.1 hypothetical protein -
KQ259_RS25025 (KQ259_25025) 50747..51865 - 1119 WP_253926428.1 hypothetical protein -
KQ259_RS25030 (KQ259_25030) 51957..52382 + 426 WP_000422741.1 transposase -
KQ259_RS25035 (KQ259_25035) 52379..52729 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
KQ259_RS25040 (KQ259_25040) 52760..54373 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / mph(A) / sul1 / qacE / aadA5 - 1..102381 102381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T206338 WP_001159868.1 NZ_CP076699:c49435-49130 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T206338 NZ_CP102374:1866996-1867289 [Pseudomonas sp. T8]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAACACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT206338 WP_000813634.1 NZ_CP076699:c49655-49437 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT206338 NZ_CP102374:1867291-1867581 [Pseudomonas sp. T8]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCACGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References