Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4997736..4998312 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag KQ259_RS24215 Protein ID WP_001295743.1
Coordinates 4997736..4998023 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag KQ259_RS24220 Protein ID WP_000063148.1
Coordinates 4998010..4998312 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS24200 (4993396) 4993396..4994700 - 1305 WP_000535012.1 restriction endonuclease subunit S -
KQ259_RS24205 (4994690) 4994690..4996309 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
KQ259_RS24210 (4996373) 4996373..4997539 - 1167 WP_000800831.1 restriction endonuclease -
KQ259_RS24215 (4997736) 4997736..4998023 + 288 WP_001295743.1 BrnT family toxin Toxin
KQ259_RS24220 (4998010) 4998010..4998312 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
KQ259_RS24225 (4998346) 4998346..4999302 - 957 WP_001295745.1 GTPase -
KQ259_RS24230 (4999313) 4999313..4999516 - 204 WP_000467859.1 YbdD/YjiX family protein -
KQ259_RS24235 (4999566) 4999566..5001716 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T206336 WP_001295743.1 NZ_CP076697:4997736-4998023 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T206336 NZ_CP102359:c5226028-5225765 [Streptomyces sp. B146]
GTGAGTGAATACCGAACCGTCTTCCGGCCAGAGGCACAGGCCGAGCTCCGGAAGATCCCTCGCGACATGGCCCTACGCAT
CCTGACCAAACTGACCGAACTGGAGAGCGACCCCCTCGGCTATAGCACCACCGCGCTCGTGTCCCAGCCAGAACGCCGTC
GACTGCGGGTCGGCGACTACCGGGTCGTCTACACGATCGACAAGGGGGAGCTCGTGGTGTGGGTCGTCCACGTCGGGCAT
CGCTCCACCGTTTACGAAGCCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT206336 WP_000063148.1 NZ_CP076697:4998010-4998312 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT206336 NZ_CP102359:c5226290-5226021 [Streptomyces sp. B146]
ATGACGCAGCCACTGCCCATAGAGTCCATCCGCGACGTACGTGCGCACCTGGCCGAGGTCGTGGAGCGCGCCGATCGGGA
TGATGTGCCCACCGTGATCACTCGACGGGGCAAGCAGATTGCCGCCGTCGTCTCCATCGAGGTGCTGCGCAAGTACCAGG
AATGGGAAGAGCGCGAGATCAATCGGATCATCGACGAACGCATGGCCAACCCGGCACCCGGCATCCCGATCGAGGAGATC
ATGAGGGAGACGCTGGCACGCGGTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References