Detailed information of TA system TA206335

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4916671..4917506
• Replicon: chromosome
• Accession: NZ_CP076697
• Organism: Escherichia coli strain S10EC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: KQ259_RS23830
• Protein ID: WP_000854759.1
• Coordinates: 4917129..4917506 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KQ259_RS23825
• Protein ID: WP_001295723.1
• Coordinates: 4916671..4917039 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ259_RS23800 (4912673)	4912673..4913146	+	474	WP_001350782.1	antirestriction protein	-
KQ259_RS23805 (4913162)	4913162..4913638	+	477	WP_001186775.1	RadC family protein	-
KQ259_RS23810 (4913701)	4913701..4913955	+	255	WP_023281719.1	DUF987 domain-containing protein	-
KQ259_RS23815 (4914096)	4914096..4915637	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
KQ259_RS23820 (4915652)	4915652..4916398	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
KQ259_RS23825 (4916671)	4916671..4917039	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KQ259_RS23830 (4917129)	4917129..4917506	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
KQ259_RS23835 (4917503)	4917503..4917991	+	489	WP_000761690.1	DUF5983 family protein	-
KQ259_RS23840 (4918008)	4918008..4918184	+	177	WP_000839286.1	DUF957 domain-containing protein	-
KQ259_RS23845 (4918290)	4918290..4918439	+	150	Protein_4689	hypothetical protein	-
KQ259_RS23850 (4918806)	4918806..4918982	+	177	Protein_4690	helix-turn-helix domain-containing protein	-
KQ259_RS23855 (4919773)	4919773..4921395	+	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T206335 WP_000854759.1 NZ_CP076697:4917129-4917506 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T206335 NZ_CP102359:4215801-4216211 [Streptomyces sp. B146]
GTGATGTACCTGCTCGATACGTCCGGCCTGGTCAGGCTGCTCCGCGATGCGAACCTGCAATCGGCCTGGCACGACGCGAT
CGACGCCGGAGCCATCGCATCCTGCTACGTGCAACGCACCGAGTTCCTGTACAGCGCCCGGAATCGCAGCGAGTACGACG
AGATCGCGGAAATGTTCTCCGACCTCTATCCCGATGCGGCGGTGCCGAAGAACGCAGGGCGTTGGATCAGCGCGGTGCAA
CACCGTATGGCTCAGGCCGGGGAGCACCGCAGTGCCTCGGCAGTAGACCTTGTCATCGCAGCCACTGCCGCGCACCACGG
CCTGACTGTCCTCCACGACGATGCCGACTACGGCACTGTCGCCCGGCACGCATCCGACCTGAACGAGCACAACGTCCACG
AAGTCGTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT206335 WP_001295723.1 NZ_CP076697:4916671-4917039 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT206335 NZ_CP102359:4215583-4215804 [Streptomyces sp. B146]
ATGTCCGTGACCCAGATCGACATCGATGACGACGCCCTGGAGCGCGCCATGGCTCTGGCCCATGTCCGGACCAAGAAGGA
GGCGGTCAATCTCGCCCTGCATTTCTACGCCGAGCAGCAGGAACGTGCGGCGCGCATCAGCCGTCACTTCGAGCGTGCCC
GCGAGTGGGGTGCCGTCGAGGACGCCGAGCGCCTGCACAGGGTGGAGAAGCGCAGCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52