Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4714270..4714767 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KQ259_RS22810 Protein ID WP_001173343.1
Coordinates 4714594..4714767 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag KQ259_RS22805 Protein ID WP_001312331.1
Coordinates 4714270..4714566 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS22795 (4709627) 4709627..4713556 + 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -
KQ259_RS22800 (4713770) 4713770..4714042 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
KQ259_RS22805 (4714270) 4714270..4714566 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KQ259_RS22810 (4714594) 4714594..4714767 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KQ259_RS22815 (4714886) 4714886..4716403 - 1518 WP_000003806.1 lysine--tRNA ligase -
KQ259_RS22820 (4716640) 4716640..4718097 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4698679..4723621 24942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T206333 WP_001173343.1 NZ_CP076697:4714594-4714767 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T206333 NZ_CP102359:2197331-2197594 [Streptomyces sp. B146]
ATGCCCATCACCGCCAGCGAAGCCCGTCAGAACCTGTTCCCGCTGATAGAGCAGGTCAACGAGGACCATGCCCCGGTACA
CATCACCTCCCGCAAGGGAAACGCCGTACTCATGTCCGAGGAGGACTTCACGGCGTGGACGGAGACGGTGCACCTCCTGC
GCTCGCCCAAGAACGCCCGCCGTCTGCTCGACTCCATCGCGGAGGCCGAAGCGGGCGAAGCACGGCATCGCGAGCTGATC
GACCCGGACGCGGAGCGGGCGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT206333 WP_001312331.1 NZ_CP076697:4714270-4714566 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT206333 NZ_CP102359:2197591-2197845 [Streptomyces sp. B146]
GTGAGGGTCACTTTCACGTCCCACGGCTGGGAGGACTACGTCCACTGGGCCGAGAGCGACCGGAAGGTGACCAAGCGGAT
CAACAGACTGATCGACGACATCACCCGTGACCCGTTCAAGGGCATCGGGAAGCCGGAGCCGCTCAAGGGCGACCTGTCGG
GCTACTGGTCACGGCGCATCGACGACACGCACCGGCTCGTGTACAAGCCCGCCGACGGCGTACTGGTCATCGTGCAGGCC
CGCTATCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References