Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4152117..4152952 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name yeeV Uniprot ID A0A376WVB1
Locus tag KQ259_RS20220 Protein ID WP_001094448.1
Coordinates 4152575..4152952 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3T7EC32
Locus tag KQ259_RS20215 Protein ID WP_024175935.1
Coordinates 4152117..4152485 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS20185 (4148615) 4148615..4149361 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
KQ259_RS20190 (4149546) 4149546..4149779 + 234 WP_001117568.1 DUF905 family protein -
KQ259_RS20195 (4149870) 4149870..4150688 + 819 WP_001234753.1 DUF932 domain-containing protein -
KQ259_RS20200 (4150780) 4150780..4151265 + 486 WP_000214415.1 antirestriction protein -
KQ259_RS20205 (4151277) 4151277..4151753 + 477 WP_001186709.1 RadC family protein -
KQ259_RS20210 (4151822) 4151822..4152043 + 222 WP_000692346.1 DUF987 domain-containing protein -
KQ259_RS20215 (4152117) 4152117..4152485 + 369 WP_024175935.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KQ259_RS20220 (4152575) 4152575..4152952 + 378 WP_001094448.1 TA system toxin CbtA family protein Toxin
KQ259_RS20225 (4152949) 4152949..4153437 + 489 WP_000761714.1 DUF5983 family protein -
KQ259_RS20230 (4153449) 4153449..4153641 + 193 Protein_3993 hypothetical protein -
KQ259_RS20235 (4153726) 4153726..4154571 + 846 WP_000065751.1 DUF4942 domain-containing protein -
KQ259_RS20240 (4155164) 4155164..4155661 + 498 WP_000509815.1 hypothetical protein -
KQ259_RS20245 (4155839) 4155839..4156762 + 924 WP_000535950.1 carboxylate/amino acid/amine transporter -
KQ259_RS20250 (4156766) 4156766..4157584 - 819 WP_000779409.1 lipoprotein NlpA -
KQ259_RS25530 (4157682) 4157682..4157933 + 252 WP_171004589.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14221.22 Da        Isoelectric Point: 8.2919

>T206331 WP_001094448.1 NZ_CP076697:4152575-4152952 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Download         Length: 378 bp

>T206331 NZ_CP102358:4339272-4339622 [Pseudomonas zeae]
ATGGATGCTCTATTTATCGAATTGCCTGTATTTCAGAAGCACCGAGACGACTATCTGGATGACGATCTGTTTCGCAGCTT
TCAACTGGAGTTGCTGAAAAATCCGGAAGCGGGCGATCTCATCGAGGACACAGGCGGGCTTCGTAAAATTCGCTTTGGCG
ACCAACGAAGAGGGAAAGGTAAGCGCAGCGGATTACGAGTGATTTATTACTGGTGGTCAGGCTTCGATCAGTTCTGGCTC
TTTACCGTCTACAACAAAAACGAGCAGGACGATTTGACGCCTGCCCAGAAAAAACTTTTCAGAAAAACGCTGGATAGAGA
ACTCAACGTGAGAACCCACTATGAAACGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13598.40 Da        Isoelectric Point: 6.6255

>AT206331 WP_024175935.1 NZ_CP076697:4152117-4152485 [Escherichia coli]
VSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT206331 NZ_CP102358:4339612-4339926 [Pseudomonas zeae]
ATGAAACGTGACATTTTTTCCGAACTGATGGATGGACTGGAAGCACTGGCCGATGAACGCCAAGGCAAAATCACCTTGCG
TACCCACAAAGTTCAACTGCCAAAACTGATGCCGATCACTGCTGAAGAAGTGATAGCCATCCGTCAGCAACTTAATCTTT
CCCGGTCAGTATTCGCGATGTATCTACGCACCAACACGCGAACGCTCGAAAACTGGGAGCAAGGACGAGCAACGCCCAAT
GCTCAGGCCACAACCCTGATCCGTCTGGTCGAGCGTTTTCCACAGACAATCGAACAACTCGCAGCCCTGACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376WVB1

Antitoxin

Source ID Structure
AlphaFold DB A0A3T7EC32

References