Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3839051..3839851 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag KQ259_RS18800 Protein ID WP_000342452.1
Coordinates 3839051..3839578 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag KQ259_RS18805 Protein ID WP_001277107.1
Coordinates 3839585..3839851 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS18775 (3834127) 3834127..3834894 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KQ259_RS18780 (3834891) 3834891..3836168 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
KQ259_RS18785 (3836165) 3836165..3837091 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KQ259_RS18790 (3837139) 3837139..3838248 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KQ259_RS18795 (3838671) 3838671..3839054 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
KQ259_RS18800 (3839051) 3839051..3839578 - 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KQ259_RS18805 (3839585) 3839585..3839851 - 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KQ259_RS18810 (3840000) 3840000..3841103 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KQ259_RS18815 (3841374) 3841374..3842228 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KQ259_RS18820 (3842473) 3842473..3843531 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
KQ259_RS18825 (3843524) 3843524..3844192 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T206330 WP_000342452.1 NZ_CP076697:c3839578-3839051 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T206330 NZ_CP102358:c2702349-2702080 [Pseudomonas zeae]
ATGCAGGTTGAGTGGCAGCCCGAAGCTCGGGCTGAACTCCTGAAAATACTTGATTACATCAGTGACAGGAACTTAGTAGC
GGCTGACAGACTTTCCCAAGCTATCGAAGGCGCTACGGTTACCCTTGCTCAACATCCACACCTCTATCGAATGGGTCGAG
CGCCCGGTACTCGCGAGATGGTCGTGCACCCCAATTACCTGATCATTTATCAAGTGGTGGGTCGGATAAATATTTTGAGC
GTTTTGCATGCGCGTCAGGAATATCCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT206330 WP_001277107.1 NZ_CP076697:c3839851-3839585 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT206330 NZ_CP102358:c2702545-2702339 [Pseudomonas zeae]
ATGATCGACCCGTCTTTAATCGACCCACTGTGCCCAGTTGTTTCAGATCTTGACCCCGAAGAGCAGGCCGCGAGCTATGA
CCGTTGGTTAATCGCCAAAGTGCAGGCTTCGATGAGTGATACAAGACCGAGCATTCCAAATGATCAGGTTATGGCCGAGA
TGCGTGCCCTGATGGAAGCAAAGCGTAAAAAATACGATGCAGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References