Detailed information of TA system TA020633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-AbrB
• Location: 3186430..3186994
• Replicon: chromosome
• Accession: NC_002977
• Organism: Methylococcus capsulatus str. Bath

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q602Q5
• Locus tag: MCA_RS14750
• Protein ID: WP_010962196.1
• Coordinates: 3186650..3186994 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q602Q6
• Locus tag: MCA_RS14745
• Protein ID: WP_010962195.1
• Coordinates: 3186430..3186660 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCA_RS14740	3182749..3186387	+	3639	WP_010962194.1	DUF3883 domain-containing protein	-
MCA_RS14745	3186430..3186660	+	231	WP_010962195.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
MCA_RS14750	3186650..3186994	+	345	WP_010962196.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MCA_RS14755	3186991..3190029	+	3039	WP_010962197.1	DUF1156 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-108)

Antitoxin

• SpoVT-AbrB domain (5-31)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12042.99 Da
• Isoelectric Point: 10.1098

>T20633 WP_010962196.1 NC_002977:3186650-3186994 [Methylococcus capsulatus str. Bath]
VTYERGQIVRVPFPFTDRAASKNRPALVLSGASAFNTPAGHVVLAMITSAKNPSWPLDCAIEDLAAAGLPAPSVVRCKLF
TLDARLIRGVLGALAETDAQRTAQTFARLLGTLP

Nucleotide

• Length: 345 bp

>T20633 NC_002977:3186650-3186994 [Methylococcus capsulatus str. Bath]
GTGACCTATGAGCGCGGCCAAATCGTGCGCGTGCCCTTCCCCTTCACCGACCGCGCCGCCAGCAAGAACCGCCCGGCGCT
GGTGCTCTCCGGCGCCAGCGCCTTCAACACCCCCGCCGGGCACGTGGTGCTGGCCATGATCACCTCGGCCAAGAACCCGT
CCTGGCCGCTGGACTGCGCCATCGAAGACCTGGCCGCCGCCGGCCTGCCCGCGCCTTCGGTGGTGCGCTGCAAGCTGTTC
ACCCTGGATGCCCGGCTCATCCGCGGGGTGCTCGGCGCGCTGGCTGAAACCGACGCGCAGCGCACGGCGCAGACCTTCGC
CCGACTTCTCGGCACCCTCCCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8275.34 Da
• Isoelectric Point: 4.3855

>AT20633 WP_010962195.1 NC_002977:3186430-3186660 [Methylococcus capsulatus str. Bath]
MTAVAKITAKGQTTIPADIRAALHVGPGDLIAWEIEPDGSARVRRVQPLDIEYLKAVEGTLSEWTSPADEEAYRDL

Nucleotide

• Length: 231 bp

>AT20633 NC_002977:3186430-3186660 [Methylococcus capsulatus str. Bath]
ATGACCGCCGTGGCCAAAATCACCGCCAAGGGCCAGACCACCATCCCGGCGGACATCCGCGCCGCGCTCCACGTCGGTCC
CGGCGACCTCATCGCCTGGGAGATCGAACCCGACGGCAGCGCCCGCGTGCGCCGTGTGCAACCGCTCGACATCGAATACC
TGAAGGCCGTCGAGGGCACCCTGTCCGAGTGGACGAGCCCGGCCGACGAGGAGGCCTACCGTGACCTATGA

Structures

Toxin

• AlphaFold DB: Q602Q5

Antitoxin

• AlphaFold DB: Q602Q6