Detailed information of TA system TA206326

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3306599..3307433
• Replicon: chromosome
• Accession: NZ_CP076697
• Organism: Escherichia coli strain S10EC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: KQ259_RS16175
• Protein ID: WP_000854689.1
• Coordinates: 3307056..3307433 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1X3JHN3
• Locus tag: KQ259_RS16170
• Protein ID: WP_001285598.1
• Coordinates: 3306599..3306979 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ259_RS16135 (3302882)	3302882..3303337	+	456	WP_000581502.1	IrmA family protein	-
KQ259_RS16140 (3303442)	3303442..3303651	+	210	WP_032142237.1	DUF905 family protein	-
KQ259_RS16145 (3303752)	3303752..3304570	+	819	WP_001234620.1	DUF932 domain-containing protein	-
KQ259_RS16150 (3304625)	3304625..3305110	+	486	WP_000849566.1	antirestriction protein	-
KQ259_RS16155 (3305126)	3305126..3305602	+	477	WP_001186726.1	RadC family protein	-
KQ259_RS16160 (3305665)	3305665..3305886	+	222	WP_000692329.1	DUF987 domain-containing protein	-
KQ259_RS16165 (3305905)	3305905..3306549	+	645	WP_000094916.1	hypothetical protein	-
KQ259_RS16170 (3306599)	3306599..3306979	+	381	WP_001285598.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KQ259_RS16175 (3307056)	3307056..3307433	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
KQ259_RS16180 (3307430)	3307430..3307918	+	489	WP_000761699.1	DUF5983 family protein	-
KQ259_RS16185 (3307935)	3307935..3308132	+	198	WP_000839293.1	DUF957 domain-containing protein	-
KQ259_RS16190 (3308217)	3308217..3309062	+	846	WP_023281696.1	DUF4942 domain-containing protein	-
KQ259_RS16195 (3309131)	3309131..3309526	+	396	WP_000208383.1	DUF6088 family protein	-
KQ259_RS16200 (3309519)	3309519..3310412	+	894	WP_001114681.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
KQ259_RS16205 (3310883)	3310883..3311053	+	171	Protein_3195	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T206326 WP_000854689.1 NZ_CP076697:3307056-3307433 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T206326 NZ_CP102358:564917-565099 [Pseudomonas zeae]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGTAGAGTTCCAAAACGGCAAGGGTAGCCACTTCAAGGT
TTCCTTGAACGGCAAATCAACTGTGTTTCCCGACCACGGAGCCAAGGAAATGGGCGAAGGGTTGAGAAAATCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13927.72 Da
• Isoelectric Point: 4.7959

>AT206326 WP_001285598.1 NZ_CP076697:3306599-3306979 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT206326 NZ_CP102358:565131-565556 [Pseudomonas zeae]
ATGTTCGAATATGCGTTGGAAGTTCACGAAGAGCCGGACAGTGTCTGGTTGTCCTGCGCAGAAATTCCAGAGATGCACGC
CGTTGGCGACACACTTGAACAGGCGCTCGACAGTGCTATCGATGCCATCGAGACCGCCCTGTCGATCTACGTTGATGATC
GTCGATTGGCTCCGACGGGGCAGGCGGGAGAACAGAAAAGTGACGTCGTGTTGCGTCTGCCTGCGCTGACCGCAGCGAAG
GTTGCGCTTTGGAATACGCTCTTGGAGTCCGGTGTGAGCAAAGCCGAACTGGCGCGTCGTCTCGGCGTGCAACGGCCTCA
GGTAGATCGGTTAGTCGATTTCCTGCATCACTCCAAGATCGAGAATGTCGAGCGTGCTTTGCAGCAGCTTGGGCGTCGGA
TTTTGCTTTCGGTTGAGGCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1X3JHN3