Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3160263..3160917 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag KQ259_RS15420 Protein ID WP_000244765.1
Coordinates 3160263..3160670 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag KQ259_RS15425 Protein ID WP_000354050.1
Coordinates 3160651..3160917 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS15400 (3156220) 3156220..3157953 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
KQ259_RS15405 (3157959) 3157959..3158669 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KQ259_RS15410 (3158694) 3158694..3159590 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KQ259_RS15415 (3159702) 3159702..3160223 + 522 WP_001055874.1 flavodoxin FldB -
KQ259_RS15420 (3160263) 3160263..3160670 - 408 WP_000244765.1 protein YgfX Toxin
KQ259_RS15425 (3160651) 3160651..3160917 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
KQ259_RS15430 (3161160) 3161160..3162140 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
KQ259_RS15435 (3162217) 3162217..3162876 - 660 WP_000250275.1 hemolysin III family protein -
KQ259_RS15440 (3163040) 3163040..3163351 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
KQ259_RS15445 (3163396) 3163396..3164829 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T206325 WP_000244765.1 NZ_CP076697:c3160670-3160263 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T206325 NZ_CP102358:503007-503189 [Pseudomonas zeae]
ATGCGCAGTCGGGAAATGATCAGGATGATCGAAGACGATGGTTGGTATCTCGTGGCGGTGAAAGGCAGCCACCACCAATA
CAAGCATCCGCACAAGCCAGGAAGGGTGACGATCAAGCACCCTGATTCGGATCTGCCCAAGGGCACGATCAACAGCATTT
TGAAACAGGCGGGCTTGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT206325 WP_000354050.1 NZ_CP076697:c3160917-3160651 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT206325 NZ_CP102358:503249-503650 [Pseudomonas zeae]
ATGAAATTCCCTGTCGTTCTGCACAAGGATGTCGACTCAGACTACGGAGTGATCATCCCGGATGTCCCGGGTTGTTTCTC
CGCAGGCACTACGGTCGCTGAGGCGTTTGAAAATACTCAGGAGGCATTGGCGCTTCACTATGAGGGGTTGGTCGCCGACA
ACGCGCCGTTACCGCAAGTGCGGGACATTGATGCTCATCTCGATAACCCGGATTACGCGGGAGGGATCTGGGGCGTGGTC
GATTTTGATATCACCCCGTATTTCGGCAAGTCAGTGCGTTTTAACGCCACGCTGCCGGAGCAGTTGCTTGAGCGTATTGA
CCAGACAGTCCGCCGTGACCAGCGCTATCGATCCCGTTCCGGATTTCTCGCGGCGGCCGCCTTGCGCGAACTGTCGGTAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References