Detailed information of TA system TA020632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2824384..2825422
• Replicon: chromosome
• Accession: NC_002977
• Organism: Methylococcus capsulatus str. Bath

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: MCA_RS12915
• Protein ID: WP_010961851.1
• Coordinates: 2824384..2824956 (-)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: Q604A7
• Locus tag: MCA_RS12920
• Protein ID: WP_010961852.1
• Coordinates: 2824961..2825422 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCA_RS12890	2819640..2820023	-	384	WP_010961846.1	hypothetical protein	-
MCA_RS12895	2820020..2821399	-	1380	WP_010961847.1	recombinase family protein	-
MCA_RS12900	2821399..2821842	-	444	WP_010961848.1	DUF2924 domain-containing protein	-
MCA_RS12905	2821842..2822033	-	192	WP_041361328.1	hypothetical protein	-
MCA_RS12910	2822119..2824365	-	2247	WP_010961850.1	hypothetical protein	-
MCA_RS12915	2824384..2824956	-	573	WP_010961851.1	PIN domain-containing protein	Toxin
MCA_RS12920	2824961..2825422	-	462	WP_010961852.1	helix-turn-helix domain-containing protein	Antitoxin
MCA_RS12925	2825651..2825914	+	264	WP_010961853.1	DNA-binding protein	-
MCA_RS12930	2825925..2826404	+	480	WP_010961854.1	hypothetical protein	-
MCA_RS12935	2826408..2827166	+	759	WP_010961855.1	Bro-N domain-containing protein	-
MCA_RS12940	2827166..2827984	+	819	WP_010961856.1	ATP-binding protein	-
MCA_RS12945	2827988..2828614	+	627	WP_010961857.1	hypothetical protein	-
MCA_RS12950	2828676..2829149	+	474	WP_010961858.1	hypothetical protein	-
MCA_RS12955	2829146..2829877	+	732	WP_041361329.1	hypothetical protein	-
MCA_RS12960	2829923..2830153	+	231	WP_143708621.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2817934..2878183	60249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• SinI-like, DNA-binding domain (83-128)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21462.57 Da
• Isoelectric Point: 5.7502

>T20632 WP_010961851.1 NC_002977:c2824956-2824384 [Methylococcus capsulatus str. Bath]
MSSHFTVVYDACVLYPAPLRDLLMHLALSDLYRARWSDMIHDEWTRNVLVSRPDLTQDQLNRTRQLMNAHVRDCLVTGFE
YLIPSIDLPDPDDRHVVAAAIHSGASLIVTFNLKDFPPEALRPYNLAAQHPDDFIVDLLDLHPAGVLEAAASHRRSLKNP
PKTADEYLDTLLAQGLTQSVAVMRQWIVAM

Nucleotide

• Length: 573 bp

>T20632 NC_002977:c2824956-2824384 [Methylococcus capsulatus str. Bath]
ATGAGTTCGCACTTCACCGTCGTCTATGACGCCTGCGTGCTCTATCCGGCACCCCTGCGCGACCTGTTGATGCATCTGGC
GCTGTCGGATCTGTACCGGGCGCGCTGGAGCGACATGATTCACGATGAGTGGACGCGCAACGTACTGGTCAGCCGTCCCG
ATCTCACTCAAGACCAGCTGAACCGGACGCGCCAGCTGATGAACGCCCACGTCCGGGATTGTCTGGTCACCGGCTTCGAG
TACCTGATTCCCTCGATCGATCTGCCCGATCCGGACGACCGCCATGTGGTGGCGGCCGCCATCCACTCCGGGGCCAGTCT
GATCGTGACCTTCAATCTCAAGGACTTCCCGCCCGAGGCGCTCAGGCCCTACAACCTCGCCGCCCAGCATCCGGACGACT
TCATCGTCGATCTGCTGGATCTGCATCCGGCAGGAGTGCTGGAGGCTGCAGCCAGCCACCGGCGATCACTGAAGAACCCG
CCCAAGACGGCGGACGAATACCTGGACACCCTGCTGGCGCAGGGGCTGACGCAATCGGTGGCGGTGATGCGCCAATGGAT
CGTGGCCATGTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16907.33 Da
• Isoelectric Point: 6.4907

>AT20632 WP_010961852.1 NC_002977:c2825422-2824961 [Methylococcus capsulatus str. Bath]
MNTPAIPKTLPSAEDIALARESGRVLSTVLQTRAETQQIDFHDEKGAVRTVSIPTSALRLLLDVLTEIGQGNAVSIIPIH
AELTTQEAADVLNVSRPFLVQLLEKGDIPFHKIGTHRRVRYQDVIAYKNRIDAERRKALDELAAQAQELGMGY

Nucleotide

• Length: 462 bp

>AT20632 NC_002977:c2825422-2824961 [Methylococcus capsulatus str. Bath]
ATGAACACTCCCGCCATCCCGAAAACGTTGCCTTCGGCCGAGGACATCGCGCTCGCCCGGGAATCCGGGCGCGTGCTCTC
GACCGTGCTCCAGACCCGTGCCGAAACCCAGCAGATCGACTTCCATGACGAAAAGGGCGCGGTGCGCACCGTATCGATCC
CGACTTCGGCGTTGCGCCTGCTGCTCGACGTCCTGACCGAGATCGGCCAGGGCAACGCCGTGTCGATCATCCCCATCCAT
GCCGAGCTGACGACGCAGGAGGCTGCCGACGTGCTCAACGTCTCGCGCCCCTTCCTCGTCCAGTTGCTGGAGAAAGGCGA
TATCCCGTTCCACAAGATCGGCACGCATCGCCGCGTGCGCTACCAGGACGTGATCGCCTACAAGAACCGGATCGACGCCG
AGCGCCGCAAGGCGCTCGATGAACTGGCGGCCCAGGCCCAGGAACTCGGCATGGGGTATTGA

Structures

Antitoxin

• AlphaFold DB: Q604A7