Detailed information of TA system TA206304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 122610..123413
• Replicon: plasmid pS103EC_A
• Accession: NZ_CP076694
• Organism: Escherichia coli strain S103EC

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: KQ261_RS26065
• Protein ID: WP_000348883.1
• Coordinates: 122610..123140 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: KQ261_RS26070
• Protein ID: WP_001275013.1
• Coordinates: 123144..123413 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ261_RS26040 (118091)	118091..118582	-	492	WP_000528930.1	HEPN family nuclease	-
KQ261_RS26045 (118973)	118973..121594	+	2622	WP_000283688.1	histidinol-phosphatase	-
KQ261_RS26050 (121793)	121793..122023	+	231	WP_001261274.1	type II toxin-antitoxin system VapB family antitoxin	-
KQ261_RS26055 (122020)	122020..122336	+	317	Protein_152	type II toxin-antitoxin system VapC family toxin	-
KQ261_RS26060 (122392)	122392..122609	-	218	Protein_153	transposase	-
KQ261_RS26065 (122610)	122610..123140	-	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
KQ261_RS26070 (123144)	123144..123413	-	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
KQ261_RS26075 (124301)	124301..125290	+	990	WP_024201093.1	RepB family plasmid replication initiator protein	-
KQ261_RS26080 (125572)	125572..126354	-	783	WP_216684396.1	tyrosine-type recombinase/integrase	-
KQ261_RS26085 (126475)	126475..126657	-	183	WP_042065278.1	hypothetical protein	-
KQ261_RS26605 (126724)	126724..127014	-	291	Protein_159	type II toxin-antitoxin system RelE/ParE family toxin	-
KQ261_RS26095 (127001)	127001..127269	-	269	Protein_160	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..146805	146805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T206304 WP_000348883.1 NZ_CP076694:c123140-122610 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T206304 NZ_CP102345:1370070-1370462 [Mycolicibacterium smegmatis]
GTGACCGAGTACCTCGACCGCGAGGACGTGCTCACCGCGGGATCGATCGCTTTTGGCGGCGAGCTGAAGGTGCGGGACTA
CGGACTGCTCGACGCTGCGGTTGCCCGCCCGCAGGCCACGGTGTATGGCGTGGATGCGTACCCGCGGCTGTGGGACAAGG
CCGCTGCGTTGTTGCAGTCCCTGGCGCGGAACCATGCGCTGGTCGACGGTAACAAGCGCACTGCGTGGGCTGCGGCATGG
ACATTTCTGCACATCAACGGTGTACAGCTGGCGGCTGATTTCGATGTCGATCGCGCGGAGGACCTCATGAACGAGGTGGC
CACCCGTGACTGCGACCTGGATTCCATCGCCGCCGAGCTGGCCGGTTTCGCCGCTGCTGCGCAGACGGGGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT206304 WP_001275013.1 NZ_CP076694:c123413-123144 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT206304 NZ_CP102345:1369876-1370073 [Mycolicibacterium smegmatis]
ATGCCGTCGCTGAACATCGACTTCGACGAAGCCGAGATGGAACAGATCCGCGCTGCTGCCCGGGCGGATGACCTGTCGCT
GAAGAAGTTCGCGCACGCCGCCGTGATGGAGCGTGCCAGCGCGCACAAGCGCAGGGTCGCCGAGGCGGCGCGTCTGGTCG
CTGAGCGTTCGGCTGAGCTCAACCGCCGCTTGGCGTGA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7