Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 100288..100889 Replicon plasmid pS103EC_A
Accession NZ_CP076694
Organism Escherichia coli strain S103EC

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag KQ261_RS25940 Protein ID WP_001216034.1
Coordinates 100509..100889 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KQ261_RS25935 Protein ID WP_001190712.1
Coordinates 100288..100509 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ261_RS25925 (97269) 97269..98552 - 1284 WP_001617890.1 restriction endonuclease subunit S -
KQ261_RS25930 (98549) 98549..100105 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
KQ261_RS25935 (100288) 100288..100509 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KQ261_RS25940 (100509) 100509..100889 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KQ261_RS25945 (100894) 100894..101073 + 180 WP_001513661.1 hypothetical protein -
KQ261_RS25950 (101101) 101101..101379 + 279 Protein_131 pdcB -
KQ261_RS25955 (101384) 101384..101797 + 414 Protein_132 integrase core domain-containing protein -
KQ261_RS25960 (101747) 101747..102082 - 336 WP_169329198.1 type I deoxyribonuclease HsdR -
KQ261_RS25965 (102292) 102292..103272 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
KQ261_RS25970 (103516) 103516..104919 + 1404 WP_001373486.1 S-methylmethionine permease -
KQ261_RS25975 (104906) 104906..105838 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..146805 146805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T206303 WP_001216034.1 NZ_CP076694:100509-100889 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T206303 NZ_CP102344:c5734226-5733783 [Mycolicibacterium smegmatis]
ATGGCCAAACTTTCCGTCTCCGTCGAAGTTCCATTGCCGCCGGAGCAGGCGTGGGAGTATGCCTCCGACCTGTCCCGGTA
CCACGAGTGGCTCAGCATTCACCGGGCCTGGCGCTCGAAGCTGCCCGAGACCCTGGAGAAGGGCACGGTGATCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGGTCAGGTGGACGTTGGTCCACTACAAGCCCCCGCAGGCGCTCACACTCAAC
GGTGACGGCCGCGGCGGGGTCAAGGTCAAGCTGATCGGCAAGATCAAGCCCACCGACAACGGCGCGACGGTGGGCTTCGA
CCTGCACCTCGGCGGCCCAGCCCTGTTCGGGCCCATCGGGATGGTGGTGGCCGCGGCGCTCAAGAGCGACATTCAGGAGT
CGCTCAACCGCTTCAAGCAGCTCTACGCCCCGTCCGCGACCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT206303 WP_001190712.1 NZ_CP076694:100288-100509 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT206303 NZ_CP102344:c5734424-5734236 [Mycolicibacterium smegmatis]
ATGGGATTCCTGGACAAGGCCAAAGACCTCTTGTCGCAGAACGCCGACAAGGTGGACACCGTCATTGACAAGGCCGGCGA
CATGATCGACGAGAAGACGCAGGGCAAGTACGCCCAGCACGTCGACAAGGCGCAGGACGCCGCGAAGAACGCCATCCACA
AAGACGACCCTCAAGAACCACAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References