Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5126744..5127320 Replicon chromosome
Accession NZ_CP076693
Organism Escherichia coli strain S103EC

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag KQ261_RS25045 Protein ID WP_001295743.1
Coordinates 5126744..5127031 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag KQ261_RS25050 Protein ID WP_000063148.1
Coordinates 5127018..5127320 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ261_RS25030 (5122404) 5122404..5123708 - 1305 WP_000535012.1 restriction endonuclease subunit S -
KQ261_RS25035 (5123698) 5123698..5125317 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
KQ261_RS25040 (5125381) 5125381..5126547 - 1167 WP_000800831.1 restriction endonuclease -
KQ261_RS25045 (5126744) 5126744..5127031 + 288 WP_001295743.1 BrnT family toxin Toxin
KQ261_RS25050 (5127018) 5127018..5127320 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
KQ261_RS25055 (5127354) 5127354..5128310 - 957 WP_001295745.1 GTPase -
KQ261_RS25060 (5128321) 5128321..5128524 - 204 WP_000467859.1 YbdD/YjiX family protein -
KQ261_RS25065 (5128574) 5128574..5130724 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T206295 WP_001295743.1 NZ_CP076693:5126744-5127031 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T206295 NZ_CP102343:1377361-1377750 [Mycolicibacterium smegmatis]
ATGGTTATCGACACTTCTGCCCTCGTTGCCATCTTGACCGACGAACCCGACGCCGAGTTGTTGGAGGGGGCGGTGGCTGA
CGATCCTGTTCGGACTATGTCCACCGCGTCGTATCTGGAGACAGCGATCGTGATCGAAAGTCGCTTCGGCGAGCCAGGTG
GTCGCGAACTCGATCTGTGGTTGCATCGCGCATCAGTGGCACTGGTCGCTGTGGATGCCGATCAGGCCGACGCCGCCCGG
TTGGCTTACCGGAGATACGGCAAGGGGCGCCATCGTGCAGGCCTGAATTACGGCGACTGTTTTTCCTATGCGCTCGCCAA
GGTCAGCGGTCAGCCTTTGTTGTTCAAGGGCGAGGCTTTTCGGCTCACCGACGTCGCTGCGGTCCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT206295 WP_000063148.1 NZ_CP076693:5127018-5127320 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT206295 NZ_CP102343:1377107-1377361 [Mycolicibacterium smegmatis]
ATGGCTCTAAGCATCAAACACCCGGAAGCCGACCGGCTGGCCAGAGAGCTAGCGGCACGTACGGGGGAGACACTGACCGA
GGCGGTGGTGATGGCACTGCGGGAGCGACTGGCGCGCACTGTCGGCCGCACTCAGGTCGTTCCGCTTCGTGAGGAACTCG
CCGCGATCCGTCGTCGCTGCGCAGCCCTACCGGTGTTGGACGACCGGACAGCCGAATCGATCCTGGGCTACGACGACCGC
GGTCTGCCGTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References