Detailed information of TA system TA206294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5045679..5046514
• Replicon: chromosome
• Accession: NZ_CP076693
• Organism: Escherichia coli strain S103EC

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: KQ261_RS24660
• Protein ID: WP_000854759.1
• Coordinates: 5046137..5046514 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KQ261_RS24655
• Protein ID: WP_001295723.1
• Coordinates: 5045679..5046047 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ261_RS24625 (5041681)	5041681..5041827	+	147	Protein_4837	antirestriction protein	-
KQ261_RS24630 (5042020)	5042020..5043561	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
KQ261_RS24635 (5043576)	5043576..5044322	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
KQ261_RS24640 (5044408)	5044408..5044740	+	333	Protein_4840	antirestriction protein	-
KQ261_RS24645 (5044756)	5044756..5045232	+	477	WP_001186775.1	RadC family protein	-
KQ261_RS24650 (5045295)	5045295..5045516	+	222	WP_000692345.1	DUF987 domain-containing protein	-
KQ261_RS24655 (5045679)	5045679..5046047	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KQ261_RS24660 (5046137)	5046137..5046514	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
KQ261_RS24665 (5046511)	5046511..5046999	+	489	WP_000761690.1	DUF5983 family protein	-
KQ261_RS24670 (5047016)	5047016..5047192	+	177	WP_000839286.1	DUF957 domain-containing protein	-
KQ261_RS24675 (5047298)	5047298..5047447	+	150	Protein_4847	hypothetical protein	-
KQ261_RS24680 (5047814)	5047814..5047990	+	177	Protein_4848	helix-turn-helix domain-containing protein	-
KQ261_RS24685 (5048781)	5048781..5050403	+	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5029398..5058160	28762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T206294 WP_000854759.1 NZ_CP076693:5046137-5046514 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T206294 NZ_CP102343:1370070-1370462 [Mycolicibacterium smegmatis]
GTGACCGAGTACCTCGACCGCGAGGACGTGCTCACCGCGGGATCGATCGCTTTTGGCGGCGAGCTGAAGGTGCGGGACTA
CGGACTGCTCGACGCTGCGGTTGCCCGCCCGCAGGCCACGGTGTATGGCGTGGATGCGTACCCGCGGCTGTGGGACAAGG
CCGCTGCGTTGTTGCAGTCCCTGGCGCGGAACCATGCGCTGGTCGACGGTAACAAGCGCACTGCGTGGGCTGCGGCATGG
ACATTTCTGCACATCAACGGTGTACAGCTGGCGGCTGATTTCGATGTCGATCGCGCGGAGGACCTCATGAACGAGGTGGC
CACCCGTGACTGCGACCTGGATTCCATCGCCGCCGAGCTGGCCGGTTTCGCCGCTGCTGCGCAGACGGGGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT206294 WP_001295723.1 NZ_CP076693:5045679-5046047 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT206294 NZ_CP102343:1369876-1370073 [Mycolicibacterium smegmatis]
ATGCCGTCGCTGAACATCGACTTCGACGAAGCCGAGATGGAACAGATCCGCGCTGCTGCCCGGGCGGATGACCTGTCGCT
GAAGAAGTTCGCGCACGCCGCCGTGATGGAGCGTGCCAGCGCGCACAAGCGCAGGGTCGCCGAGGCGGCGCGTCTGGTCG
CTGAGCGTTCGGCTGAGCTCAACCGCCGCTTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52