Detailed information of TA system TA020627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 1900273..1900802
• Replicon: chromosome
• Accession: NC_002971
• Organism: Coxiella burnetii RSA 493

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q83AB3
• Locus tag: CBU_1992
• Protein ID: NP_820967.1
• Coordinates: 1900527..1900802 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q83AB4
• Locus tag: CBU_1991
• Protein ID: NP_820966.1
• Coordinates: 1900273..1900527 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBU_1984	1895341..1895715	+	375	NP_820959.1	hypothetical protein	-
CBU_1985	1895760..1896458	+	699	NP_820960.1	hypothetical protein	-
CBU_1986	1896522..1897463	+	942	NP_820961.1	signal peptide peptidase	-
CBU_1987	1897476..1898351	+	876	NP_820962.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
CBU_1987a	1898500..1898634	+	135	YP_002333045.1	hypothetical protein	-
CBU_1987b	1898795..1899814	+	1020	YP_002333046.2	IS110 family transposase IS1111A	-
CBU_1987c	1899811..1900083	-	273	YP_002333047.1	hypothetical protein	-
CBU_1991	1900273..1900527	+	255	NP_820966.1	toxin-antitoxin system antitoxin RelB	Antitoxin
CBU_1992	1900527..1900802	+	276	NP_820967.1	toxin-antitoxin system antitoxin RelE	Toxin
CBU_1993	1900775..1901260	-	486	NP_820968.1	dihydrofolate reductase	-
CBU_1994	1901257..1902060	-	804	NP_820969.2	hypothetical protein	-
CBU_1995	1902256..1902543	+	288	NP_820970.2	acylphosphatase	-
CBU_1996	1902519..1903073	-	555	NP_820971.1	phosphatase family protein	-
CBU_1997	1903086..1904030	-	945	NP_820972.1	methionyl-tRNA formyltransferase	-
CBU_1998	1904218..1905144	+	927	NP_820973.1	DNA processing protein	-
CBU_1999	1905141..1905620	+	480	NP_820974.2	Smg protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1898795..1899814	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11150.86 Da
• Isoelectric Point: 9.6762

>T20627 NP_820967.1 NC_002971:1900527-1900802 [Coxiella burnetii RSA 493]
MQISFTPEAWEDYLYWQKFDKKMLRRINELIKDAMHEPFSGKGKPEPLKFELQGYWSRRLDQEHRLVYKVLDDSLMIIAA
RFHYNRLNSKN

Nucleotide

• Length: 276 bp

>T20627 NC_002971:1900527-1900802 [Coxiella burnetii RSA 493]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9624.25 Da
• Isoelectric Point: 10.4802

>AT20627 NP_820966.1 NC_002971:1900273-1900527 [Coxiella burnetii RSA 493]
MNVVTFSELRAQLKKILDLSADQHEPVVVKRPNKETMVILSLRDFEALKETAYLLSNEANAARLRQSIRSLKQGKAQKKK
LMED

Nucleotide

• Length: 255 bp

>AT20627 NC_002971:1900273-1900527 [Coxiella burnetii RSA 493]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Structures

Toxin

• AlphaFold DB: Q83AB3

Antitoxin

• AlphaFold DB: Q83AB4