Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55748..56268 Replicon chromosome
Accession NZ_CP076693
Organism Escherichia coli strain S103EC

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag KQ261_RS00255 Protein ID WP_001160964.1
Coordinates 55984..56268 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KQ261_RS00250 Protein ID WP_000125566.1
Coordinates 55748..55981 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ261_RS00225 (50821) 50821..51108 + 288 WP_000203744.1 ferredoxin-like protein FixX -
KQ261_RS00230 (51167) 51167..52498 + 1332 WP_001183209.1 MFS transporter -
KQ261_RS00235 (52606) 52606..53136 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KQ261_RS00240 (53129) 53129..54991 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
KQ261_RS00245 (55183) 55183..55662 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
KQ261_RS00250 (55748) 55748..55981 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KQ261_RS00255 (55984) 55984..56268 + 285 WP_001160964.1 CcdB family protein Toxin
KQ261_RS00260 (56295) 56295..57143 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KQ261_RS00265 (57150) 57150..57527 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KQ261_RS00270 (57530) 57530..58351 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KQ261_RS00275 (58348) 58348..59337 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KQ261_RS00280 (59337) 59337..60623 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T206265 WP_001160964.1 NZ_CP076693:55984-56268 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T206265 NZ_CP102267:4422597-4422887 [Blautia wexlerae DSM 19850]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAGGACTACAAACGAGCTATAAAGCGTGGCTTGAA
AATTGAGCTGTTGGAGCAGGTCGTAGCATTGCTGGCAATGGGCGAACCGCTGCCAGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT206265 WP_000125566.1 NZ_CP076693:55748-55981 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT206265 NZ_CP102267:4422322-4422600 [Blautia wexlerae DSM 19850]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCACAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTGTTTGCTGACCTGAAAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References