Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 10827..11352 Replicon plasmid pS21EC_B
Accession NZ_CP076692
Organism Escherichia coli strain S21EC

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KQ260_RS25500 Protein ID WP_001159871.1
Coordinates 10827..11132 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KQ260_RS25505 Protein ID WP_000813634.1
Coordinates 11134..11352 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ260_RS25470 (KQ260_25425) 6094..7364 + 1271 Protein_9 translesion error-prone DNA polymerase V subunit UmuC -
KQ260_RS25475 (KQ260_25430) 7369..7761 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
KQ260_RS25480 (KQ260_25435) 7766..8737 - 972 WP_001103694.1 plasmid segregation protein ParM -
KQ260_RS25485 (KQ260_25440) 8966..9610 + 645 WP_000633911.1 AAA family ATPase -
KQ260_RS25490 (KQ260_25445) 9604..9879 + 276 WP_000239529.1 hypothetical protein -
KQ260_RS25495 (KQ260_25450) 10017..10826 - 810 WP_000016979.1 site-specific integrase -
KQ260_RS25500 (KQ260_25455) 10827..11132 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KQ260_RS25505 (KQ260_25460) 11134..11352 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KQ260_RS25510 (KQ260_25465) 13674..14315 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..25878 25878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T206264 WP_001159871.1 NZ_CP076692:c11132-10827 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T206264 NZ_CP102267:c2251505-2251230 [Blautia wexlerae DSM 19850]
ATGAAATATACGGTTAAGCCGACCAGTAAGTTTCAAAAAGACTTAAAACGTATTCAGAAACGTGGATACGATATGAGATT
AATGACTGATATCATCAAAAAATTGGCAAATGGTGAAATATTACCGCCGAAGAATCGAGATCATAATCTTTCAGGAAATT
ATAGTAATTGCAGAGAATGTCATATTGCTCCTGATTGGTTGTTGATTTATGAAGTCTATGAAGATGAACTATTCCTATAT
TTGACAAGAACTGGAAGTCATAGTGATTTGTTTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT206264 WP_000813634.1 NZ_CP076692:c11352-11134 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT206264 NZ_CP102267:c2251771-2251502 [Blautia wexlerae DSM 19850]
ATGGCAAATGTATCAATTAGAATGGATGATAATTTAAAGAAACAAGCAGAAGATCTTTTCAATGATTTAGGTATGAATCT
GACAACTGCATTCACAATTTTTGTAAAACAGGCAATCAGAGAACAGGGAATACCGTTTGAGATTACCAGAGAAATACCAA
ACAGTGAAACAATATCAGCAATTAATGAAGTGCAGCAGATGAAACAGAATCCTTCTCTTGGAAAAGCATATACAGATGTG
GACAAGATGATGGAGGAATTATTAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References